Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury
Hypoxia/reoxygenation (H/R)-induced injury is the key factor associated with islet graft dysfunction. This study aims to examine the effect of mesenchymal stem cells (MSCs) on islet survival and insulin secretion under H/R conditions. Islets from rats were isolated, purified, cultured with or withou...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/3539 |
| _version_ | 1848744259682828288 |
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| author | Lu, Y. Jin, X. Chen, Younan Li, S. Yuan, Y. Mai, G. Tian, B. Long, D. Zhang, J. Zeng, L. Li, Y. Cheng, J. |
| author_facet | Lu, Y. Jin, X. Chen, Younan Li, S. Yuan, Y. Mai, G. Tian, B. Long, D. Zhang, J. Zeng, L. Li, Y. Cheng, J. |
| author_sort | Lu, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Hypoxia/reoxygenation (H/R)-induced injury is the key factor associated with islet graft dysfunction. This study aims to examine the effect of mesenchymal stem cells (MSCs) on islet survival and insulin secretion under H/R conditions. Islets from rats were isolated, purified, cultured with or without MSCs, and exposed to hypoxia (O2 = 1%) for 8 h and reoxygenation for 24 and 48 h, respectively. Islet function was evaluated by measuring basal and glucose-stimulated insulin secretion (GSIS). Apoptotic islet cells were quantified using Annexin V-FITC. Anti-apoptotic effects were confirmed by mRNA expression analysis of hypoxia-resistant molecules, HIF-1a, HO-1, and COX-2, using semi-quantitative retrieval polymerase chain reaction (RT-PCR). Insulin expression in the implanted islets was detected by immunohistological analysis. The main results show that the stimulation index (SI) of GSIS was maintained at higher levels in islets co-cultured with MSCs. The MSCs protected the islets from H/R-induced injury by decreasing the apoptotic cell ratio and increasing HIF-1a, HO-1, and COX-2 mRNA expression. Seven days after islet transplantation, insulin expression in the MSC-islets group significantly differed from that of the islets-alone group. We proposed that MSCs could promote anti-apoptotic gene expression by enhancing their resistance to H/R-induced apoptosis and dysfunction. This study provides an experimental basis for therapeutic strategies based on enhancing islet function. © 2010 John Wiley & Sons, Ltd. |
| first_indexed | 2025-11-14T05:58:38Z |
| format | Journal Article |
| id | curtin-20.500.11937-3539 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T05:58:38Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-35392017-09-13T14:44:37Z Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury Lu, Y. Jin, X. Chen, Younan Li, S. Yuan, Y. Mai, G. Tian, B. Long, D. Zhang, J. Zeng, L. Li, Y. Cheng, J. Hypoxia/reoxygenation (H/R)-induced injury is the key factor associated with islet graft dysfunction. This study aims to examine the effect of mesenchymal stem cells (MSCs) on islet survival and insulin secretion under H/R conditions. Islets from rats were isolated, purified, cultured with or without MSCs, and exposed to hypoxia (O2 = 1%) for 8 h and reoxygenation for 24 and 48 h, respectively. Islet function was evaluated by measuring basal and glucose-stimulated insulin secretion (GSIS). Apoptotic islet cells were quantified using Annexin V-FITC. Anti-apoptotic effects were confirmed by mRNA expression analysis of hypoxia-resistant molecules, HIF-1a, HO-1, and COX-2, using semi-quantitative retrieval polymerase chain reaction (RT-PCR). Insulin expression in the implanted islets was detected by immunohistological analysis. The main results show that the stimulation index (SI) of GSIS was maintained at higher levels in islets co-cultured with MSCs. The MSCs protected the islets from H/R-induced injury by decreasing the apoptotic cell ratio and increasing HIF-1a, HO-1, and COX-2 mRNA expression. Seven days after islet transplantation, insulin expression in the MSC-islets group significantly differed from that of the islets-alone group. We proposed that MSCs could promote anti-apoptotic gene expression by enhancing their resistance to H/R-induced apoptosis and dysfunction. This study provides an experimental basis for therapeutic strategies based on enhancing islet function. © 2010 John Wiley & Sons, Ltd. 2010 Journal Article http://hdl.handle.net/20.500.11937/3539 10.1002/cbf.1701 restricted |
| spellingShingle | Lu, Y. Jin, X. Chen, Younan Li, S. Yuan, Y. Mai, G. Tian, B. Long, D. Zhang, J. Zeng, L. Li, Y. Cheng, J. Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| title | Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| title_full | Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| title_fullStr | Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| title_full_unstemmed | Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| title_short | Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| title_sort | mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury |
| url | http://hdl.handle.net/20.500.11937/3539 |