Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia
Sequences representing the genomes of two distinct virus isolates infecting wild plants of two members of the genus Drakaea (hammer orchids) in Western Australia are described. The virus isolated from Drakaea livida has a bipartite genome of 4490 nt (RNA1) and 2905 nt (RNA2) that shares closest sequ...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Blackwell Publishing Ltd
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/35234 |
| _version_ | 1848754441332719616 |
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| author | Ong, J. Phillips, R. Dixon, Kingsley Jones, M. Wylie, S. |
| author_facet | Ong, J. Phillips, R. Dixon, Kingsley Jones, M. Wylie, S. |
| author_sort | Ong, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Sequences representing the genomes of two distinct virus isolates infecting wild plants of two members of the genus Drakaea (hammer orchids) in Western Australia are described. The virus isolated from Drakaea livida has a bipartite genome of 4490 nt (RNA1) and 2905 nt (RNA2) that shares closest sequence and structural similarity to members of the genus Pecluvirus, family Virgaviridae, described from legumes in the Indian subcontinent and West Africa. However, it differs from pecluviruses by lacking a P39 protein on RNA2 and having a cysteine-rich protein gene located 3′ of the triple gene block protein genes. It is the first peclu-like virus to be described from Australia. The name Drakaea virus A is proposed (DVA; proposed member of the family Virgaviridae, genus unassigned). The second virus isolate was identified from Drakaea elastica, a species classed as endangered under conservation legislation. The genome sequence of this virus shares closest identity with isolates of Donkey orchid symptomless virus (DOSV; proposed member of the order Tymovirales, family and genus unassigned), a species described previously from wild Caladenia and Diuris orchids in the same region. These viruses are the first to be isolated from wild Drakaea populations and are proposed to have an ancient association with their orchid hosts. |
| first_indexed | 2025-11-14T08:40:27Z |
| format | Journal Article |
| id | curtin-20.500.11937-35234 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:40:27Z |
| publishDate | 2016 |
| publisher | Blackwell Publishing Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-352342017-09-13T15:33:15Z Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia Ong, J. Phillips, R. Dixon, Kingsley Jones, M. Wylie, S. Sequences representing the genomes of two distinct virus isolates infecting wild plants of two members of the genus Drakaea (hammer orchids) in Western Australia are described. The virus isolated from Drakaea livida has a bipartite genome of 4490 nt (RNA1) and 2905 nt (RNA2) that shares closest sequence and structural similarity to members of the genus Pecluvirus, family Virgaviridae, described from legumes in the Indian subcontinent and West Africa. However, it differs from pecluviruses by lacking a P39 protein on RNA2 and having a cysteine-rich protein gene located 3′ of the triple gene block protein genes. It is the first peclu-like virus to be described from Australia. The name Drakaea virus A is proposed (DVA; proposed member of the family Virgaviridae, genus unassigned). The second virus isolate was identified from Drakaea elastica, a species classed as endangered under conservation legislation. The genome sequence of this virus shares closest identity with isolates of Donkey orchid symptomless virus (DOSV; proposed member of the order Tymovirales, family and genus unassigned), a species described previously from wild Caladenia and Diuris orchids in the same region. These viruses are the first to be isolated from wild Drakaea populations and are proposed to have an ancient association with their orchid hosts. 2016 Journal Article http://hdl.handle.net/20.500.11937/35234 10.1111/ppa.12396 Blackwell Publishing Ltd restricted |
| spellingShingle | Ong, J. Phillips, R. Dixon, Kingsley Jones, M. Wylie, S. Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia |
| title | Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia |
| title_full | Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia |
| title_fullStr | Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia |
| title_full_unstemmed | Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia |
| title_short | Characterization of the first two viruses described from wild populations of hammer orchids (Drakaea spp.) in Australia |
| title_sort | characterization of the first two viruses described from wild populations of hammer orchids (drakaea spp.) in australia |
| url | http://hdl.handle.net/20.500.11937/35234 |