Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep
Corneodesmosin (CDSN) is an important component of the desmosome in the epidermal cornified stratum and inner root sheath of hair follicles. DNA from a sheep BAC clone previously identified by us to contain CDSN was PCR amplified using cattle-derived primers and the product sequenced. A region of 45...
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| Format: | Journal Article |
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Wiley-Blackwell
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/35174 |
| _version_ | 1848754423927406592 |
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| author | Siva Subramaniam, Nitthiya Morgan, Eleanor Bottomley, Steve Tay, S. Gregg, Keith Lee, Chee Yang Wetherall, John Groth, David |
| author_facet | Siva Subramaniam, Nitthiya Morgan, Eleanor Bottomley, Steve Tay, S. Gregg, Keith Lee, Chee Yang Wetherall, John Groth, David |
| author_sort | Siva Subramaniam, Nitthiya |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Corneodesmosin (CDSN) is an important component of the desmosome in the epidermal cornified stratum and inner root sheath of hair follicles. DNA from a sheep BAC clone previously identified by us to contain CDSN was PCR amplified using cattle-derived primers and the product sequenced. A region of 4579 bp containing CDSN was shown to contain two exons separated by one intron and spanning 3683 bp. The DNA encodes a predicted protein of 546 amino acids. Phylogenetic analysis shows that sheep CDSN falls within a clade containing cattle and other ruminant-like species. Comparison of sequences generated from 12 unrelated merino sheep and the International Sheep Genome Consortium (ISGC) data identified 58 single nucleotide polymorphisms (SNPs) within the 4579 bp region of which 16 are contained within coding sequences (1 in 80 bp). The SNPs identified in this study will add to the Major Histocompatibility Complex (MHC) SNP panel, which will allow extensive haplotyping of the sheep MHC in future studies. |
| first_indexed | 2025-11-14T08:40:11Z |
| format | Journal Article |
| id | curtin-20.500.11937-35174 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:40:11Z |
| publishDate | 2012 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-351742017-09-13T16:07:46Z Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep Siva Subramaniam, Nitthiya Morgan, Eleanor Bottomley, Steve Tay, S. Gregg, Keith Lee, Chee Yang Wetherall, John Groth, David genetics corneodesmosin polymorphism sheep MHC Corneodesmosin (CDSN) is an important component of the desmosome in the epidermal cornified stratum and inner root sheath of hair follicles. DNA from a sheep BAC clone previously identified by us to contain CDSN was PCR amplified using cattle-derived primers and the product sequenced. A region of 4579 bp containing CDSN was shown to contain two exons separated by one intron and spanning 3683 bp. The DNA encodes a predicted protein of 546 amino acids. Phylogenetic analysis shows that sheep CDSN falls within a clade containing cattle and other ruminant-like species. Comparison of sequences generated from 12 unrelated merino sheep and the International Sheep Genome Consortium (ISGC) data identified 58 single nucleotide polymorphisms (SNPs) within the 4579 bp region of which 16 are contained within coding sequences (1 in 80 bp). The SNPs identified in this study will add to the Major Histocompatibility Complex (MHC) SNP panel, which will allow extensive haplotyping of the sheep MHC in future studies. 2012 Journal Article http://hdl.handle.net/20.500.11937/35174 10.1111/j.1740-0929.2011.00975.x Wiley-Blackwell restricted |
| spellingShingle | genetics corneodesmosin polymorphism sheep MHC Siva Subramaniam, Nitthiya Morgan, Eleanor Bottomley, Steve Tay, S. Gregg, Keith Lee, Chee Yang Wetherall, John Groth, David Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep |
| title | Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep |
| title_full | Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep |
| title_fullStr | Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep |
| title_full_unstemmed | Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep |
| title_short | Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep |
| title_sort | predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (cdsn) gene in sheep |
| topic | genetics corneodesmosin polymorphism sheep MHC |
| url | http://hdl.handle.net/20.500.11937/35174 |