Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents
PHEMA-peptide and P[HEMA-co-(MeO-PEGMA)]-peptide conjugate hydrogels [where PHEMA ) poly(2-hydroxyethyl methacrylate; PEGMA ) poly(ethylene glycol) methacrylate] were readily prepared via photoinitiated free-radical polymerization in water. The PHEMA-peptide hydrogels were opaque and had a heterogen...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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American Chemical Society
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/34842 |
| _version_ | 1848754333011673088 |
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| author | Casadio, Y. Brown, David Chirila, T. Kraatz, H. Baker, M. |
| author_facet | Casadio, Y. Brown, David Chirila, T. Kraatz, H. Baker, M. |
| author_sort | Casadio, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | PHEMA-peptide and P[HEMA-co-(MeO-PEGMA)]-peptide conjugate hydrogels [where PHEMA ) poly(2-hydroxyethyl methacrylate; PEGMA ) poly(ethylene glycol) methacrylate] were readily prepared via photoinitiated free-radical polymerization in water. The PHEMA-peptide hydrogels were opaque and had a heterogeneous morphology of interconnected polymer droplets, characteristic of polymers that separate from the aqueous phase during the polymerization experiment. The P[HEMA-co-(MeO-PEGMA)]-peptide conjugates were transparent gels with a homogeneous morphology when formed in water, but when formed in aqueous NaCl solutions the P[HEMA-co-(MeO-PEGMA)]-peptide conjugates were also opaque and exhibited the heterogeneous morphology of interconnected polymer droplets. When incubated in solutions containing activated papain, P[HEMA-co-(MeOPEGMA)]- peptide conjugates underwent degradation that was characterized by macroscopic changes to sample shape and size, sample weight, and microscopic structure. PHEMA-peptide conjugates did not undergo any significant degradation when incubated with papain, although ninhydrin-staining experiments suggested that some peptide cross-linker groups were cleaved during the incubation. The difference in degradation behavior of PHEMA-peptide and P[HEMA-co-(MeO-PEGMA)]-peptide conjugates is attributed to differences in aqueous solubility of PHEMA and P[HEMA-co-(MeO-PEGMA)]. |
| first_indexed | 2025-11-14T08:38:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-34842 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:38:44Z |
| publishDate | 2010 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-348422017-09-13T15:53:33Z Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents Casadio, Y. Brown, David Chirila, T. Kraatz, H. Baker, M. PHEMA-peptide and P[HEMA-co-(MeO-PEGMA)]-peptide conjugate hydrogels [where PHEMA ) poly(2-hydroxyethyl methacrylate; PEGMA ) poly(ethylene glycol) methacrylate] were readily prepared via photoinitiated free-radical polymerization in water. The PHEMA-peptide hydrogels were opaque and had a heterogeneous morphology of interconnected polymer droplets, characteristic of polymers that separate from the aqueous phase during the polymerization experiment. The P[HEMA-co-(MeO-PEGMA)]-peptide conjugates were transparent gels with a homogeneous morphology when formed in water, but when formed in aqueous NaCl solutions the P[HEMA-co-(MeO-PEGMA)]-peptide conjugates were also opaque and exhibited the heterogeneous morphology of interconnected polymer droplets. When incubated in solutions containing activated papain, P[HEMA-co-(MeOPEGMA)]- peptide conjugates underwent degradation that was characterized by macroscopic changes to sample shape and size, sample weight, and microscopic structure. PHEMA-peptide conjugates did not undergo any significant degradation when incubated with papain, although ninhydrin-staining experiments suggested that some peptide cross-linker groups were cleaved during the incubation. The difference in degradation behavior of PHEMA-peptide and P[HEMA-co-(MeO-PEGMA)]-peptide conjugates is attributed to differences in aqueous solubility of PHEMA and P[HEMA-co-(MeO-PEGMA)]. 2010 Journal Article http://hdl.handle.net/20.500.11937/34842 10.1021/bm100756c American Chemical Society restricted |
| spellingShingle | Casadio, Y. Brown, David Chirila, T. Kraatz, H. Baker, M. Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents |
| title | Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents |
| title_full | Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents |
| title_fullStr | Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents |
| title_full_unstemmed | Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents |
| title_short | Biodegradation of Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} Hydrogels ContainingPeptide-Based Cross-Linking Agents |
| title_sort | biodegradation of poly(2-hydroxyethyl methacrylate) (phema) and poly{(2-hydroxyethyl methacrylate)-co-[poly(ethylene glycol) methyl ether methacrylate]} hydrogels containingpeptide-based cross-linking agents |
| url | http://hdl.handle.net/20.500.11937/34842 |