A new organic carbon detector for size exclusion chromatography.
A novel organic carbon detector (OCD) for size exclusion chromatography (SEC), and its application to the characterisation of aquatic natural organic matter (NOM) in natural and treated potable water samples, is described. The instrument uses a conventional UV-persulfate oxidation technique to conve...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Elsevier
2007
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/3477 |
| _version_ | 1848744242771394560 |
|---|---|
| author | Allpike, Bradley Heitz, Anna Joll, Cynthia Kagi, Robert |
| author_facet | Allpike, Bradley Heitz, Anna Joll, Cynthia Kagi, Robert |
| author_sort | Allpike, Bradley |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A novel organic carbon detector (OCD) for size exclusion chromatography (SEC), and its application to the characterisation of aquatic natural organic matter (NOM) in natural and treated potable water samples, is described. The instrument uses a conventional UV-persulfate oxidation technique to convert organic carbon to CO2. The novelty of the technique is detection of the evolved CO2 using a sensitive Fourier transform infrared (FTIR) spectroscopy 'lightpipe' detector originally designed for detection of analytes after gas chromatographic separation. With the exception of the lightpipe, the OCD system was constructed using simple, inexpensive, readily available components. The system was designed to minimise deadvolume, allowing for use of smaller sample sizes and smaller columns, substantially shortening analysis time, while maintaining chromatographic integrity through the OCD system. Downscaling resulted in some loss of separation but it was shown that this was caused by thelower separation efficiency of the smaller capacity column, rather than from sample dispersion within the OCD system. |
| first_indexed | 2025-11-14T05:58:21Z |
| format | Journal Article |
| id | curtin-20.500.11937-3477 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T05:58:21Z |
| publishDate | 2007 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-34772017-09-13T16:06:40Z A new organic carbon detector for size exclusion chromatography. Allpike, Bradley Heitz, Anna Joll, Cynthia Kagi, Robert Size exclusion chromatography (SEC) Natural organic matter (NOM) Organic carbon detector Fourier transform infrared spectroscopy (FTIR) Water Dissolved organic carbon (DOC) A novel organic carbon detector (OCD) for size exclusion chromatography (SEC), and its application to the characterisation of aquatic natural organic matter (NOM) in natural and treated potable water samples, is described. The instrument uses a conventional UV-persulfate oxidation technique to convert organic carbon to CO2. The novelty of the technique is detection of the evolved CO2 using a sensitive Fourier transform infrared (FTIR) spectroscopy 'lightpipe' detector originally designed for detection of analytes after gas chromatographic separation. With the exception of the lightpipe, the OCD system was constructed using simple, inexpensive, readily available components. The system was designed to minimise deadvolume, allowing for use of smaller sample sizes and smaller columns, substantially shortening analysis time, while maintaining chromatographic integrity through the OCD system. Downscaling resulted in some loss of separation but it was shown that this was caused by thelower separation efficiency of the smaller capacity column, rather than from sample dispersion within the OCD system. 2007 Journal Article http://hdl.handle.net/20.500.11937/3477 10.1016/j.chroma.2007.05.073 Elsevier restricted |
| spellingShingle | Size exclusion chromatography (SEC) Natural organic matter (NOM) Organic carbon detector Fourier transform infrared spectroscopy (FTIR) Water Dissolved organic carbon (DOC) Allpike, Bradley Heitz, Anna Joll, Cynthia Kagi, Robert A new organic carbon detector for size exclusion chromatography. |
| title | A new organic carbon detector for size exclusion chromatography. |
| title_full | A new organic carbon detector for size exclusion chromatography. |
| title_fullStr | A new organic carbon detector for size exclusion chromatography. |
| title_full_unstemmed | A new organic carbon detector for size exclusion chromatography. |
| title_short | A new organic carbon detector for size exclusion chromatography. |
| title_sort | new organic carbon detector for size exclusion chromatography. |
| topic | Size exclusion chromatography (SEC) Natural organic matter (NOM) Organic carbon detector Fourier transform infrared spectroscopy (FTIR) Water Dissolved organic carbon (DOC) |
| url | http://hdl.handle.net/20.500.11937/3477 |