The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia
Between 2003 and 2008, 76 clinical isolates of the Panton-Valentine leukocidin-positive Staphylococcus aureus strain 'West Australian methicillin-resistant Staphylococcus aureus (MRSA)-12'(WA MRSA-12) were recovered from 72 patients living in the Perth area in Western Australia. These isol...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Blackwell Publishing
2009
|
| Online Access: | http://hdl.handle.net/20.500.11937/34691 |
| _version_ | 1848754292372013056 |
|---|---|
| author | Monecke, S. Ehricht, R. Slickers, P. Tan, H. Coombs, Geoffrey |
| author_facet | Monecke, S. Ehricht, R. Slickers, P. Tan, H. Coombs, Geoffrey |
| author_sort | Monecke, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Between 2003 and 2008, 76 clinical isolates of the Panton-Valentine leukocidin-positive Staphylococcus aureus strain 'West Australian methicillin-resistant Staphylococcus aureus (MRSA)-12'(WA MRSA-12) were recovered from 72 patients living in the Perth area in Western Australia. These isolates were found to belong to multilocus sequence type 8, and had a USA300-like pulsed-field gel electrophoresis pulsotype. All isolates were genotyped using diagnostic DNA arrays covering species markers, resistance factors, virulence-associated, as well as MSCRAMM (microbial surface components recognizing adhesive matrix molecules) genes to prove the identity between WA MRSA-12 and the pandemic strain USA300, as well as to detect possible genetic variability. In general, WA MRSA-12 isolates were similar to USA300, and the most common variant was identical to USA300- TC1516 . From this clone, most of the other variants may have evolved by a limited number of gene losses or acquisitions. Variations in carriage of virulence and resistance-associated genes allow distinction of variants or sub-clones. Altogether, 16 variants could be distinguished. They differed in the carriage of resistance genes (blaZ/I/R, ermC, msrA+mpbBM, aadD+mupR, aphA3+sat, tetK, qacC, merA/B/R/T) of ß-haemolysin-converting phages and of enterotoxins (sek + seq, which were deleted in four isolates). Notably, the arginine catabolic mobile element (ACME) was absent in 12 isolates (15.8%). The mercury resistance (mer) operon, which is usually associated with SCC mec type III elements, was found in several ACME-negative isolates. The present study emphasises the importance of genotyping in detecting the introduction and evolution of significant MRSA strains within a community. © 2009 The Authors Journal compilation © 2009 European Society of Clinical Microbiology and Infectious Diseases. |
| first_indexed | 2025-11-14T08:38:05Z |
| format | Journal Article |
| id | curtin-20.500.11937-34691 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:38:05Z |
| publishDate | 2009 |
| publisher | Blackwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-346912017-09-13T15:22:41Z The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia Monecke, S. Ehricht, R. Slickers, P. Tan, H. Coombs, Geoffrey Between 2003 and 2008, 76 clinical isolates of the Panton-Valentine leukocidin-positive Staphylococcus aureus strain 'West Australian methicillin-resistant Staphylococcus aureus (MRSA)-12'(WA MRSA-12) were recovered from 72 patients living in the Perth area in Western Australia. These isolates were found to belong to multilocus sequence type 8, and had a USA300-like pulsed-field gel electrophoresis pulsotype. All isolates were genotyped using diagnostic DNA arrays covering species markers, resistance factors, virulence-associated, as well as MSCRAMM (microbial surface components recognizing adhesive matrix molecules) genes to prove the identity between WA MRSA-12 and the pandemic strain USA300, as well as to detect possible genetic variability. In general, WA MRSA-12 isolates were similar to USA300, and the most common variant was identical to USA300- TC1516 . From this clone, most of the other variants may have evolved by a limited number of gene losses or acquisitions. Variations in carriage of virulence and resistance-associated genes allow distinction of variants or sub-clones. Altogether, 16 variants could be distinguished. They differed in the carriage of resistance genes (blaZ/I/R, ermC, msrA+mpbBM, aadD+mupR, aphA3+sat, tetK, qacC, merA/B/R/T) of ß-haemolysin-converting phages and of enterotoxins (sek + seq, which were deleted in four isolates). Notably, the arginine catabolic mobile element (ACME) was absent in 12 isolates (15.8%). The mercury resistance (mer) operon, which is usually associated with SCC mec type III elements, was found in several ACME-negative isolates. The present study emphasises the importance of genotyping in detecting the introduction and evolution of significant MRSA strains within a community. © 2009 The Authors Journal compilation © 2009 European Society of Clinical Microbiology and Infectious Diseases. 2009 Journal Article http://hdl.handle.net/20.500.11937/34691 10.1111/j.1469-0691.2009.02792.x Blackwell Publishing unknown |
| spellingShingle | Monecke, S. Ehricht, R. Slickers, P. Tan, H. Coombs, Geoffrey The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia |
| title | The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia |
| title_full | The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia |
| title_fullStr | The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia |
| title_full_unstemmed | The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia |
| title_short | The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia |
| title_sort | molecular epidemiology and evolution of the panton-valentine leukocidin-positive, methicillin-resistant staphylococcus aureus strain usa300 in western australia |
| url | http://hdl.handle.net/20.500.11937/34691 |