Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes
Human tympanic membrane cells (hTMCs), harvested from tympanic membrane (TM) explants, were grown in culture and then seeded on membranes prepared from silkworm (Bombyx mori) silk fibroin (BMSF) and on tissue-culture plastic membranes (PET). Fibroin was isolated from silk cast into membranes with a...
| Main Authors: | , , , |
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| Format: | Journal Article |
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Sage Publications
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/34502 |
| _version_ | 1848754240402489344 |
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| author | Ghassemifar, Reza Redmond, S. Zainuddin Chirila, T. |
| author_facet | Ghassemifar, Reza Redmond, S. Zainuddin Chirila, T. |
| author_sort | Ghassemifar, Reza |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Human tympanic membrane cells (hTMCs), harvested from tympanic membrane (TM) explants, were grown in culture and then seeded on membranes prepared from silkworm (Bombyx mori) silk fibroin (BMSF) and on tissue-culture plastic membranes (PET). Fibroin was isolated from silk cast into membranes with a thickness of 10-15 µm. The hTMCs were cultured on both materials for 15 days in a serum-containing culture medium. The cells grown on both substrata were subjected to nuclear staining (DAPI) and counted. Further, the cultures were immunostained for a number of protein markers related to the epithelial/keratinocyte phenotype and cell adhesion complexes. The BMSF membranes supported levels of hTMC growth higher than that observed on the PET membranes. The immunofluorochemical analysis indicated unequivocally that BMSF is a more suitable substratum than PET with respect to the growth patterns, proliferation, and cell-cell contact and adhesion. BMSF appear as a promising substratum in the tissue-engineered constructs for the replacement of TM in case of nonhealing perforations. |
| first_indexed | 2025-11-14T08:37:16Z |
| format | Journal Article |
| id | curtin-20.500.11937-34502 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:37:16Z |
| publishDate | 2010 |
| publisher | Sage Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-345022017-09-13T15:15:01Z Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes Ghassemifar, Reza Redmond, S. Zainuddin Chirila, T. Human tympanic membrane cells (hTMCs), harvested from tympanic membrane (TM) explants, were grown in culture and then seeded on membranes prepared from silkworm (Bombyx mori) silk fibroin (BMSF) and on tissue-culture plastic membranes (PET). Fibroin was isolated from silk cast into membranes with a thickness of 10-15 µm. The hTMCs were cultured on both materials for 15 days in a serum-containing culture medium. The cells grown on both substrata were subjected to nuclear staining (DAPI) and counted. Further, the cultures were immunostained for a number of protein markers related to the epithelial/keratinocyte phenotype and cell adhesion complexes. The BMSF membranes supported levels of hTMC growth higher than that observed on the PET membranes. The immunofluorochemical analysis indicated unequivocally that BMSF is a more suitable substratum than PET with respect to the growth patterns, proliferation, and cell-cell contact and adhesion. BMSF appear as a promising substratum in the tissue-engineered constructs for the replacement of TM in case of nonhealing perforations. 2010 Journal Article http://hdl.handle.net/20.500.11937/34502 10.1177/0885328209104289 Sage Publications restricted |
| spellingShingle | Ghassemifar, Reza Redmond, S. Zainuddin Chirila, T. Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes |
| title | Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes |
| title_full | Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes |
| title_fullStr | Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes |
| title_full_unstemmed | Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes |
| title_short | Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes |
| title_sort | advancing towards a tissue-engineered tympanic membrane: silk fibroin as a substratum for growing human eardrum keratinocytes |
| url | http://hdl.handle.net/20.500.11937/34502 |