Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity

Introduction: Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previousl...

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Main Authors: Yong, H., Murthi, P., Borg, A., Kalionis, B., Moses, Eric, Brennecke, S., Keogh, R.
Format: Journal Article
Published: 2014
Online Access:http://hdl.handle.net/20.500.11937/3434
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author Yong, H.
Murthi, P.
Borg, A.
Kalionis, B.
Moses, Eric
Brennecke, S.
Keogh, R.
author_facet Yong, H.
Murthi, P.
Borg, A.
Kalionis, B.
Moses, Eric
Brennecke, S.
Keogh, R.
author_sort Yong, H.
building Curtin Institutional Repository
collection Online Access
description Introduction: Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1, INHA, INHBB, ERAP1, ERAP2, LNPEP, COL4A1 and COL4A2. The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods: Third trimester decidual tissues were collected from both normotensive (n = 21) and SPE pregnancies (n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann–Whitney U test and Spearman's Correlation. Results: The data demonstrate significantly increased decidual mRNA expression levels of ACVR1, INHBB, ERAP1, ERAP2, LNPEP, COL4A1 and COL4A2 in SPE (p < 0.05). Increased mRNA expression levels of several genes – INHA, INHBB, COL4A1 and COL4A2 were correlated with earlier onset of PE and earlier delivery of the fetus (p < 0.05). Conclusion: These results suggest altered expression of maternal susceptibility genes may play roles in PE development and the course of disease severity.
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spelling curtin-20.500.11937-34342018-03-29T09:05:22Z Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity Yong, H. Murthi, P. Borg, A. Kalionis, B. Moses, Eric Brennecke, S. Keogh, R. Introduction: Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1, INHA, INHBB, ERAP1, ERAP2, LNPEP, COL4A1 and COL4A2. The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods: Third trimester decidual tissues were collected from both normotensive (n = 21) and SPE pregnancies (n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann–Whitney U test and Spearman's Correlation. Results: The data demonstrate significantly increased decidual mRNA expression levels of ACVR1, INHBB, ERAP1, ERAP2, LNPEP, COL4A1 and COL4A2 in SPE (p < 0.05). Increased mRNA expression levels of several genes – INHA, INHBB, COL4A1 and COL4A2 were correlated with earlier onset of PE and earlier delivery of the fetus (p < 0.05). Conclusion: These results suggest altered expression of maternal susceptibility genes may play roles in PE development and the course of disease severity. 2014 Journal Article http://hdl.handle.net/20.500.11937/3434 10.1016/j.placenta.2013.11.008 restricted
spellingShingle Yong, H.
Murthi, P.
Borg, A.
Kalionis, B.
Moses, Eric
Brennecke, S.
Keogh, R.
Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
title Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
title_full Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
title_fullStr Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
title_full_unstemmed Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
title_short Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
title_sort increased decidual mrna expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
url http://hdl.handle.net/20.500.11937/3434