Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application
The extent of exothermicity associated with the construction of large-volume methacrylate monolithic columns has somewhat obstructed the realisation of large-scale rapid biomolecule purification especially for plasmid-based products which have proven to herald future trends in biotechnology. A novel...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
Elsevier BV
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/34063 |
| _version_ | 1848754119907475456 |
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| author | Danquah, Michael Forde, G. |
| author_facet | Danquah, Michael Forde, G. |
| author_sort | Danquah, Michael |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The extent of exothermicity associated with the construction of large-volume methacrylate monolithic columns has somewhat obstructed the realisation of large-scale rapid biomolecule purification especially for plasmid-based products which have proven to herald future trends in biotechnology. A novel synthesis technique via a heat expulsion mechanism was employed to prepare a 40 mL methacrylate monolith with a homogeneous radial pore structure along its thickness. Radial temperature gradient was recorded to be only 1.8 °C. Maximum radial temperature recorded at the centre of the monolith was 62.3 °C, which was only 2.3 °C higher than the actual polymerisation temperature. Pore characterisation of the monolithic polymer showed unimodal pore size distributions at different radial positions with an identical modal pore size of 400 nm. Chromatographic characterisation of the polymer after functionalisation with amino groups displayed a persistent dynamic binding capacity of 15.5 mg of plasmid DNA/mL. The maximum pressure drop recorded was only 0.12 MPa at a flow rate of 10 mL/min. The polymer demonstrated rapid separation ability by fractionating Escherichia coli DH5a-pUC19 clarified lysate in only 3 min after loading. The plasmid sample collected after the fast purification process was tested to be a homogeneous supercoiled plasmid with DNA electrophoresis and restriction analysis. © 2008 Elsevier B.V. All rights reserved. |
| first_indexed | 2025-11-14T08:35:21Z |
| format | Journal Article |
| id | curtin-20.500.11937-34063 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:35:21Z |
| publishDate | 2008 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-340632017-09-13T15:10:07Z Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application Danquah, Michael Forde, G. The extent of exothermicity associated with the construction of large-volume methacrylate monolithic columns has somewhat obstructed the realisation of large-scale rapid biomolecule purification especially for plasmid-based products which have proven to herald future trends in biotechnology. A novel synthesis technique via a heat expulsion mechanism was employed to prepare a 40 mL methacrylate monolith with a homogeneous radial pore structure along its thickness. Radial temperature gradient was recorded to be only 1.8 °C. Maximum radial temperature recorded at the centre of the monolith was 62.3 °C, which was only 2.3 °C higher than the actual polymerisation temperature. Pore characterisation of the monolithic polymer showed unimodal pore size distributions at different radial positions with an identical modal pore size of 400 nm. Chromatographic characterisation of the polymer after functionalisation with amino groups displayed a persistent dynamic binding capacity of 15.5 mg of plasmid DNA/mL. The maximum pressure drop recorded was only 0.12 MPa at a flow rate of 10 mL/min. The polymer demonstrated rapid separation ability by fractionating Escherichia coli DH5a-pUC19 clarified lysate in only 3 min after loading. The plasmid sample collected after the fast purification process was tested to be a homogeneous supercoiled plasmid with DNA electrophoresis and restriction analysis. © 2008 Elsevier B.V. All rights reserved. 2008 Journal Article http://hdl.handle.net/20.500.11937/34063 10.1016/j.chroma.2008.02.045 Elsevier BV restricted |
| spellingShingle | Danquah, Michael Forde, G. Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application |
| title | Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application |
| title_full | Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application |
| title_fullStr | Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application |
| title_full_unstemmed | Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application |
| title_short | Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application |
| title_sort | large-volume methacrylate monolith for plasmid purification. process engineering approach to synthesis and application |
| url | http://hdl.handle.net/20.500.11937/34063 |