Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system
Identification of functional programmable mechanical stimulation (PMS) on tendon not only provides the insight of the tendon homeostasis under physical/pathological condition, but also guides a better engineering strategy for tendon regeneration. The aims of the study are to design a bioreactor syst...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Wiley
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/33841 |
| _version_ | 1848754058910760960 |
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| author | Wang, Tao Lin, Zhen Day, Robert Gardiner, Bruce Landao-Bassonga, Euphemie Rubenson, Jonas Kirk, Brett Smith, David Lloyd, David Hardisty, Gerard Wang, Allan Zheng, Qiujian Zheng, Ming Hao |
| author_facet | Wang, Tao Lin, Zhen Day, Robert Gardiner, Bruce Landao-Bassonga, Euphemie Rubenson, Jonas Kirk, Brett Smith, David Lloyd, David Hardisty, Gerard Wang, Allan Zheng, Qiujian Zheng, Ming Hao |
| author_sort | Wang, Tao |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Identification of functional programmable mechanical stimulation (PMS) on tendon not only provides the insight of the tendon homeostasis under physical/pathological condition, but also guides a better engineering strategy for tendon regeneration. The aims of the study are to design a bioreactor system with PMS to mimic the in vivo loading conditions, and to define the impact of different cyclic tensile strain on tendon. Rabbit Achilles tendons were loaded in the bioreactor with/without cyclic tensile loading (0.25 Hz for 8 h/day, 0–9% for 6 days). Tendons without loading lost its structure integrity as evidenced by disorientated collagen fiber, increased type III collagen expression, and increased cell apoptosis. Tendons with 3% of cyclic tensile loading had moderate matrix deterioration and elevated expression levels of MMP-1, 3, and 12, whilst exceeded loading regime of 9% caused massive rupture of collagen bundle. However, 6% of cyclic tensile strain was able to maintain the structural integrity and cellular function. Our data indicated that an optimal PMS is required to maintain the tendon homeostasis and there is only a narrow range of tensile strain that can induce the anabolic action. The clinical impact of this study is that optimized eccentric training program is needed to achieve maximum beneficial effects on chronic tendinopathy management. |
| first_indexed | 2025-11-14T08:34:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-33841 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:34:23Z |
| publishDate | 2013 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-338412017-09-13T15:32:25Z Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system Wang, Tao Lin, Zhen Day, Robert Gardiner, Bruce Landao-Bassonga, Euphemie Rubenson, Jonas Kirk, Brett Smith, David Lloyd, David Hardisty, Gerard Wang, Allan Zheng, Qiujian Zheng, Ming Hao programmable mechanical stimulation collagen tendon bioreactor Identification of functional programmable mechanical stimulation (PMS) on tendon not only provides the insight of the tendon homeostasis under physical/pathological condition, but also guides a better engineering strategy for tendon regeneration. The aims of the study are to design a bioreactor system with PMS to mimic the in vivo loading conditions, and to define the impact of different cyclic tensile strain on tendon. Rabbit Achilles tendons were loaded in the bioreactor with/without cyclic tensile loading (0.25 Hz for 8 h/day, 0–9% for 6 days). Tendons without loading lost its structure integrity as evidenced by disorientated collagen fiber, increased type III collagen expression, and increased cell apoptosis. Tendons with 3% of cyclic tensile loading had moderate matrix deterioration and elevated expression levels of MMP-1, 3, and 12, whilst exceeded loading regime of 9% caused massive rupture of collagen bundle. However, 6% of cyclic tensile strain was able to maintain the structural integrity and cellular function. Our data indicated that an optimal PMS is required to maintain the tendon homeostasis and there is only a narrow range of tensile strain that can induce the anabolic action. The clinical impact of this study is that optimized eccentric training program is needed to achieve maximum beneficial effects on chronic tendinopathy management. 2013 Journal Article http://hdl.handle.net/20.500.11937/33841 10.1002/bit.24809 Wiley restricted |
| spellingShingle | programmable mechanical stimulation collagen tendon bioreactor Wang, Tao Lin, Zhen Day, Robert Gardiner, Bruce Landao-Bassonga, Euphemie Rubenson, Jonas Kirk, Brett Smith, David Lloyd, David Hardisty, Gerard Wang, Allan Zheng, Qiujian Zheng, Ming Hao Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| title | Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| title_full | Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| title_fullStr | Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| title_full_unstemmed | Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| title_short | Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| title_sort | programmable mechanical stimulation influences tendon homeostasis in a bioreactor system |
| topic | programmable mechanical stimulation collagen tendon bioreactor |
| url | http://hdl.handle.net/20.500.11937/33841 |