Photoaffinity labeling of plasma proteins
Photoaffinity labeling is a powerful technique for identifying a target protein. A high degree of labeling specificity can be achieved with this method in comparison to chemical labeling. Human serum albumin (HSA) and α1-acid glycoprotein (AGP) are two plasma proteins that bind a variety of endogeno...
| Main Authors: | , |
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| Format: | Journal Article |
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M D P I AG
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/33694 |
| _version_ | 1848754018067677184 |
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| author | Chuang, Victor Otagiri, M. |
| author_facet | Chuang, Victor Otagiri, M. |
| author_sort | Chuang, Victor |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Photoaffinity labeling is a powerful technique for identifying a target protein. A high degree of labeling specificity can be achieved with this method in comparison to chemical labeling. Human serum albumin (HSA) and α1-acid glycoprotein (AGP) are two plasma proteins that bind a variety of endogenous and exogenous substances. The ligand binding mechanism of these two proteins is complex. Fatty acids, which are known to be transported in plasma by HSA, cause conformational changes and participate in allosteric ligand binding to HSA. HSA undergoes an N-B transition, a conformational change at alkaline pH, that has been reported to result in increased ligand binding. Attempts have been made to investigate the impact of fatty acids and the N-B transition on ligand binding in HSA using ketoprofen and flunitrazepam as photolabeling agents. Meanwhile, plasma AGP is a mixture of genetic variants of the protein. The photolabeling of AGP with flunitrazepam has been utilized to shed light on the topology of the protein ligand binding site. Furthermore, a review of photoaffinity labeling performed on other major plasma proteins will also be discussed. Using a photoreactive natural ligand as a photolabeling agent to identify target protein in the plasma would reduce non-specific labeling. |
| first_indexed | 2025-11-14T08:33:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-33694 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:33:44Z |
| publishDate | 2013 |
| publisher | M D P I AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-336942017-09-13T15:33:14Z Photoaffinity labeling of plasma proteins Chuang, Victor Otagiri, M. protein binding labeling specificity photoreactivity human serum albumin photoaffinity labeling a1-acid glycoprotein plasma proteins Photoaffinity labeling is a powerful technique for identifying a target protein. A high degree of labeling specificity can be achieved with this method in comparison to chemical labeling. Human serum albumin (HSA) and α1-acid glycoprotein (AGP) are two plasma proteins that bind a variety of endogenous and exogenous substances. The ligand binding mechanism of these two proteins is complex. Fatty acids, which are known to be transported in plasma by HSA, cause conformational changes and participate in allosteric ligand binding to HSA. HSA undergoes an N-B transition, a conformational change at alkaline pH, that has been reported to result in increased ligand binding. Attempts have been made to investigate the impact of fatty acids and the N-B transition on ligand binding in HSA using ketoprofen and flunitrazepam as photolabeling agents. Meanwhile, plasma AGP is a mixture of genetic variants of the protein. The photolabeling of AGP with flunitrazepam has been utilized to shed light on the topology of the protein ligand binding site. Furthermore, a review of photoaffinity labeling performed on other major plasma proteins will also be discussed. Using a photoreactive natural ligand as a photolabeling agent to identify target protein in the plasma would reduce non-specific labeling. 2013 Journal Article http://hdl.handle.net/20.500.11937/33694 10.3390/molecules181113831 M D P I AG fulltext |
| spellingShingle | protein binding labeling specificity photoreactivity human serum albumin photoaffinity labeling a1-acid glycoprotein plasma proteins Chuang, Victor Otagiri, M. Photoaffinity labeling of plasma proteins |
| title | Photoaffinity labeling of plasma proteins |
| title_full | Photoaffinity labeling of plasma proteins |
| title_fullStr | Photoaffinity labeling of plasma proteins |
| title_full_unstemmed | Photoaffinity labeling of plasma proteins |
| title_short | Photoaffinity labeling of plasma proteins |
| title_sort | photoaffinity labeling of plasma proteins |
| topic | protein binding labeling specificity photoreactivity human serum albumin photoaffinity labeling a1-acid glycoprotein plasma proteins |
| url | http://hdl.handle.net/20.500.11937/33694 |