Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)

Nine microsatellite loci were identified in the western rock lobster (Panulirus cygnus) using two different methods. The first method involved the screening of a small, fragment, partial genomic library with a radioactive (CA) 6 probe. The second method, was based upon an enr...

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Main Authors: Groth, David, Lim, F., de Lestang, S., Beale, N., Melville-Smith, R.
Format: Journal Article
Published: Springer 2009
Online Access:http://hdl.handle.net/20.500.11937/33361
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author Groth, David
Lim, F.
de Lestang, S.
Beale, N.
Melville-Smith, R.
author_facet Groth, David
Lim, F.
de Lestang, S.
Beale, N.
Melville-Smith, R.
author_sort Groth, David
building Curtin Institutional Repository
collection Online Access
description Nine microsatellite loci were identified in the western rock lobster (Panulirus cygnus) using two different methods. The first method involved the screening of a small, fragment, partial genomic library with a radioactive (CA) 6 probe. The second method, was based upon an enrichment method and used biotinylated, tetranucleotide microsatellite oligonucleotide capture probes. The nine loci described are all very polymorphic, with 11 to 34 alleles observed for each locus and heterozygosities ranging from 0.58 to 0.86. These microsatellite loci will be useful in analysing both the population structure and the mating systems used by this species and will add important information for the management of the wild stocks of this economically important species. © Springer Science+Business Media B.V. 2009.
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institution Curtin University Malaysia
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publishDate 2009
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spelling curtin-20.500.11937-333612017-09-13T15:31:40Z Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus) Groth, David Lim, F. de Lestang, S. Beale, N. Melville-Smith, R. Nine microsatellite loci were identified in the western rock lobster (Panulirus cygnus) using two different methods. The first method involved the screening of a small, fragment, partial genomic library with a radioactive (CA) 6 probe. The second method, was based upon an enrichment method and used biotinylated, tetranucleotide microsatellite oligonucleotide capture probes. The nine loci described are all very polymorphic, with 11 to 34 alleles observed for each locus and heterozygosities ranging from 0.58 to 0.86. These microsatellite loci will be useful in analysing both the population structure and the mating systems used by this species and will add important information for the management of the wild stocks of this economically important species. © Springer Science+Business Media B.V. 2009. 2009 Journal Article http://hdl.handle.net/20.500.11937/33361 10.1007/s12686-009-9039-2 Springer restricted
spellingShingle Groth, David
Lim, F.
de Lestang, S.
Beale, N.
Melville-Smith, R.
Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
title Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
title_full Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
title_fullStr Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
title_full_unstemmed Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
title_short Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
title_sort characterization of polymorphic microsatellite loci in the western rock lobster (panulirus cygnus)
url http://hdl.handle.net/20.500.11937/33361