OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila
Ornithine lipids (OLs) are phosphorus-free membrane lipids widespread in bacteria but absent from archaea and eukaryotes. In addition to the unmodified OLs, a variety of OL derivatives hydroxylated in different structural positions has been reported. Recently, methylated derivatives of OLs were desc...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
American Society for Biochemistry and Molecular Biology Inc.
2015
|
| Online Access: | http://hdl.handle.net/20.500.11937/33359 |
| _version_ | 1848753924200202240 |
|---|---|
| author | Escobedo-Hinojosa, W. Vences-Guzmán, M. Schubotz, F. Sandoval-Calderón, M. Summons, Roger López-Lara, I. Geiger, O. Sohlenkamp, C. |
| author_facet | Escobedo-Hinojosa, W. Vences-Guzmán, M. Schubotz, F. Sandoval-Calderón, M. Summons, Roger López-Lara, I. Geiger, O. Sohlenkamp, C. |
| author_sort | Escobedo-Hinojosa, W. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Ornithine lipids (OLs) are phosphorus-free membrane lipids widespread in bacteria but absent from archaea and eukaryotes. In addition to the unmodified OLs, a variety of OL derivatives hydroxylated in different structural positions has been reported. Recently, methylated derivatives of OLs were described in several planctomycetes isolated from a peat bog in Northern Russia, although the gene/enzyme responsible for the N-methylation of OL remained obscure. Here we identify and characterize the OL N-methyltransferase OlsG (Sinac-1600) from the planctomycete Singulisphaera acidiphila. When OlsG is co-expressed with the OL synthase OlsF in Escherichia coli, methylated OL derivatives are formed. An in vitro characterization shows that OlsG is responsible for the 3-fold methylation of the terminal d-nitrogen of OL. Methylation is dependent on the presence of the detergent Triton X-100 and the methyldonor S-adenosylmethionine. |
| first_indexed | 2025-11-14T08:32:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-33359 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:32:14Z |
| publishDate | 2015 |
| publisher | American Society for Biochemistry and Molecular Biology Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-333592017-09-13T15:31:40Z OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila Escobedo-Hinojosa, W. Vences-Guzmán, M. Schubotz, F. Sandoval-Calderón, M. Summons, Roger López-Lara, I. Geiger, O. Sohlenkamp, C. Ornithine lipids (OLs) are phosphorus-free membrane lipids widespread in bacteria but absent from archaea and eukaryotes. In addition to the unmodified OLs, a variety of OL derivatives hydroxylated in different structural positions has been reported. Recently, methylated derivatives of OLs were described in several planctomycetes isolated from a peat bog in Northern Russia, although the gene/enzyme responsible for the N-methylation of OL remained obscure. Here we identify and characterize the OL N-methyltransferase OlsG (Sinac-1600) from the planctomycete Singulisphaera acidiphila. When OlsG is co-expressed with the OL synthase OlsF in Escherichia coli, methylated OL derivatives are formed. An in vitro characterization shows that OlsG is responsible for the 3-fold methylation of the terminal d-nitrogen of OL. Methylation is dependent on the presence of the detergent Triton X-100 and the methyldonor S-adenosylmethionine. 2015 Journal Article http://hdl.handle.net/20.500.11937/33359 10.1074/jbc.M115.639575 American Society for Biochemistry and Molecular Biology Inc. unknown |
| spellingShingle | Escobedo-Hinojosa, W. Vences-Guzmán, M. Schubotz, F. Sandoval-Calderón, M. Summons, Roger López-Lara, I. Geiger, O. Sohlenkamp, C. OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila |
| title | OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila |
| title_full | OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila |
| title_fullStr | OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila |
| title_full_unstemmed | OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila |
| title_short | OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila |
| title_sort | olsg (sinac-1600) is an ornithine lipid n-methyltransferase from the planctomycete singulisphaera acidiphila |
| url | http://hdl.handle.net/20.500.11937/33359 |