Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits
Background: Candidal meningitis is a common clinical manifestation of invasive candidiasis in neonates. The aim of this study was to evaluate the in vivo antifungal efficacy of CG3R6TAT nanoparticles, novel core-shell structures self-assembled from cationic antimicrobial peptides, in a rabbit model...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Karger
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/33298 |
| _version_ | 1848753906760286208 |
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| author | Xu, K. Wang, H. Liu, Lihong Xu, W. Sheng, J. Fan, W. Yang, Y. Li, L. |
| author_facet | Xu, K. Wang, H. Liu, Lihong Xu, W. Sheng, J. Fan, W. Yang, Y. Li, L. |
| author_sort | Xu, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Background: Candidal meningitis is a common clinical manifestation of invasive candidiasis in neonates. The aim of this study was to evaluate the in vivo antifungal efficacy of CG3R6TAT nanoparticles, novel core-shell structures self-assembled from cationic antimicrobial peptides, in a rabbit model of candidal meningitis. Methods: In vitro activity of CG3R6TAT nanoparticles against Candida albicans was assessed by determining the minimum inhibitory concentration and kill-time curves. In vivo, intravenous treatment with CG3R6TAT nanoparticles (n = 6; 0.25 mg/kg/day) or fluconazole (n = 6; 100 mg/kg/day) began 3 days after infection and continued for 11 consecutive days; the efficacy was assessed following 11 days of treatment by yeast counting in cerebrospinal fluid (CSF), the leukocyte concentrations in CSF and the histopathology of brain parenchyma. Results: At a concentration three times higher than the minimum inhibitory concentration (8.1 µmol/l), the nanoparticles completely sterilized C. albicans after 5 h of incubation. In addition, there was a significant reduction in fungal counts and leukocyte concentrations in the CSF from rabbits treated with CG3R6TAT nanoparticles or fluconazole versus those from untreated control rabbits (p < 0.05, ANCOVA). The median number of days of treatment required to sterilize CSF cultures was 8.5 days for CG3R6TAT nanoparticle therapy (p = 0.022, vs. control) and 9.7 days for fluconazole therapy (p > 0.05, vs. control). The histopathologic severity of rabbits was significantly attenuated after CG3R6TAT treatment (p = 0.001, vs. control). Conclusion: This study suggests that CG3R6TAT nanoparticles may be a promising therapeutic agent for candidal meningitis. |
| first_indexed | 2025-11-14T08:31:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-33298 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:31:58Z |
| publishDate | 2011 |
| publisher | Karger |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-332982017-09-13T16:08:35Z Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits Xu, K. Wang, H. Liu, Lihong Xu, W. Sheng, J. Fan, W. Yang, Y. Li, L. Background: Candidal meningitis is a common clinical manifestation of invasive candidiasis in neonates. The aim of this study was to evaluate the in vivo antifungal efficacy of CG3R6TAT nanoparticles, novel core-shell structures self-assembled from cationic antimicrobial peptides, in a rabbit model of candidal meningitis. Methods: In vitro activity of CG3R6TAT nanoparticles against Candida albicans was assessed by determining the minimum inhibitory concentration and kill-time curves. In vivo, intravenous treatment with CG3R6TAT nanoparticles (n = 6; 0.25 mg/kg/day) or fluconazole (n = 6; 100 mg/kg/day) began 3 days after infection and continued for 11 consecutive days; the efficacy was assessed following 11 days of treatment by yeast counting in cerebrospinal fluid (CSF), the leukocyte concentrations in CSF and the histopathology of brain parenchyma. Results: At a concentration three times higher than the minimum inhibitory concentration (8.1 µmol/l), the nanoparticles completely sterilized C. albicans after 5 h of incubation. In addition, there was a significant reduction in fungal counts and leukocyte concentrations in the CSF from rabbits treated with CG3R6TAT nanoparticles or fluconazole versus those from untreated control rabbits (p < 0.05, ANCOVA). The median number of days of treatment required to sterilize CSF cultures was 8.5 days for CG3R6TAT nanoparticle therapy (p = 0.022, vs. control) and 9.7 days for fluconazole therapy (p > 0.05, vs. control). The histopathologic severity of rabbits was significantly attenuated after CG3R6TAT treatment (p = 0.001, vs. control). Conclusion: This study suggests that CG3R6TAT nanoparticles may be a promising therapeutic agent for candidal meningitis. 2011 Journal Article http://hdl.handle.net/20.500.11937/33298 10.1159/000330855 Karger restricted |
| spellingShingle | Xu, K. Wang, H. Liu, Lihong Xu, W. Sheng, J. Fan, W. Yang, Y. Li, L. Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits |
| title | Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits |
| title_full | Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits |
| title_fullStr | Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits |
| title_full_unstemmed | Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits |
| title_short | Efficacy of CG 3 R 6 TAT Nanoparticles Self-Assembled from a Novel Antimicrobial Peptide for theTreatment of Candida albicans Meningitis in Rabbits |
| title_sort | efficacy of cg 3 r 6 tat nanoparticles self-assembled from a novel antimicrobial peptide for thetreatment of candida albicans meningitis in rabbits |
| url | http://hdl.handle.net/20.500.11937/33298 |