Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol
The effect of p-nitrophenol (PNP) concentration with or without glucose and yeast extract on the growth and biodegradative capacity of Ralstonia eutropha was examined. The chemical constituents of the culture medium were modeled using a response surface methodology. The experimentswere performed acc...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Springer
2010
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| Online Access: | http://download.springer.com/static/pdf/940/art%253A10.1007%252Fs10532-010-9332-5.pdf?auth66=1421130901_34430b27917dafba3b508520e1128056&ext=.pdf http://hdl.handle.net/20.500.11937/33286 |
| _version_ | 1848753903177302016 |
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| author | Salehi, Z. Vahabzadeh, F. Sohrabi, M. Fatemi, S. Znad, Hussein |
| author_facet | Salehi, Z. Vahabzadeh, F. Sohrabi, M. Fatemi, S. Znad, Hussein |
| author_sort | Salehi, Z. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The effect of p-nitrophenol (PNP) concentration with or without glucose and yeast extract on the growth and biodegradative capacity of Ralstonia eutropha was examined. The chemical constituents of the culture medium were modeled using a response surface methodology. The experimentswere performed according to the central composite design arrangement considering PNP,glucose and yeast extract as the selected variables whose influences on the degradation was evaluated (shaking in reciprocal mode, temperature of 30C, pH 7 and test time of about 9 h). Quadratic polynomial regression equations were used to quantitatively explain variations between and within the models (responses: the biodegradation capacity and the biomass formation). The coefficient of determination was high (Radjusted 2 = 0.9783), indicating the constructed polynomial model for PNP biodegradative capacity explains the variation between the regressors fairly well. A PNP removal efficiency of 74.5% occurred within 9 h (15 mg/L as the initial concentration of PNP with use of yeast extract at 0.5 g/L). |
| first_indexed | 2025-11-14T08:31:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-33286 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:31:54Z |
| publishDate | 2010 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-332862017-09-13T15:30:51Z Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol Salehi, Z. Vahabzadeh, F. Sohrabi, M. Fatemi, S. Znad, Hussein Biodegradation Yeast extract Central composite design Glucose Ralstonia eutropha p-nitrophenol The effect of p-nitrophenol (PNP) concentration with or without glucose and yeast extract on the growth and biodegradative capacity of Ralstonia eutropha was examined. The chemical constituents of the culture medium were modeled using a response surface methodology. The experimentswere performed according to the central composite design arrangement considering PNP,glucose and yeast extract as the selected variables whose influences on the degradation was evaluated (shaking in reciprocal mode, temperature of 30C, pH 7 and test time of about 9 h). Quadratic polynomial regression equations were used to quantitatively explain variations between and within the models (responses: the biodegradation capacity and the biomass formation). The coefficient of determination was high (Radjusted 2 = 0.9783), indicating the constructed polynomial model for PNP biodegradative capacity explains the variation between the regressors fairly well. A PNP removal efficiency of 74.5% occurred within 9 h (15 mg/L as the initial concentration of PNP with use of yeast extract at 0.5 g/L). 2010 Journal Article http://hdl.handle.net/20.500.11937/33286 10.1007/s10532-010-9332-5 http://download.springer.com/static/pdf/940/art%253A10.1007%252Fs10532-010-9332-5.pdf?auth66=1421130901_34430b27917dafba3b508520e1128056&ext=.pdf Springer restricted |
| spellingShingle | Biodegradation Yeast extract Central composite design Glucose Ralstonia eutropha p-nitrophenol Salehi, Z. Vahabzadeh, F. Sohrabi, M. Fatemi, S. Znad, Hussein Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol |
| title | Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol |
| title_full | Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol |
| title_fullStr | Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol |
| title_full_unstemmed | Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol |
| title_short | Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol |
| title_sort | statistical medium optimization and biodegradative capacity of ralstonia eutropha toward p-nitrophenol |
| topic | Biodegradation Yeast extract Central composite design Glucose Ralstonia eutropha p-nitrophenol |
| url | http://download.springer.com/static/pdf/940/art%253A10.1007%252Fs10532-010-9332-5.pdf?auth66=1421130901_34430b27917dafba3b508520e1128056&ext=.pdf http://hdl.handle.net/20.500.11937/33286 |