Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A
Neutralizing antibodies (inhibitors) to replacement factor VIII (FVIII, either plasma derived or recombinant) impair the effective management of hemophilia A1. Individuals with hemophilia A due to major deletions of the FVIII gene (F8) lack antigenically cross-reactive material in their plasma (“CRM...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
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2013
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| Online Access: | http://hdl.handle.net/20.500.11937/33146 |
| _version_ | 1848753864428224512 |
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| author | Pandey, G. Yanover, C. Miller-Jenkins, L. Garfield, S. Cole, S. Curran, J. Moses, Eric Rydz, N. Simhadri, V. Kimchi-Sarfaty, C. Lillicrap, D. Viel, K. Przytycka, T. Pierce, G. Howard, T. Sauna, Z. Lusher, J. Chitlur, M. Ameri, A. Natarajan, K. Iyer, R. Thompson, A. Watts, R. Kempton, C. Kessler, C. Barrett, J. Martin, E. Key, N. Kruse-Jarres, R. Lessinger, C. Pratt, K. Josephson, N. McRedmond, K. Withycombe, J. Walsh, C. Matthews, D. Mahlangu, J. Krause, A. Schwyzer, R. Thejpal, R. Rapiti, N. Goga, Y. Coetzee, M. Stones, D. Mann, K. Butenas, S. Almasy, L. Blangero, J. Carless, M. Raja, R. Reed, E. |
| author_facet | Pandey, G. Yanover, C. Miller-Jenkins, L. Garfield, S. Cole, S. Curran, J. Moses, Eric Rydz, N. Simhadri, V. Kimchi-Sarfaty, C. Lillicrap, D. Viel, K. Przytycka, T. Pierce, G. Howard, T. Sauna, Z. Lusher, J. Chitlur, M. Ameri, A. Natarajan, K. Iyer, R. Thompson, A. Watts, R. Kempton, C. Kessler, C. Barrett, J. Martin, E. Key, N. Kruse-Jarres, R. Lessinger, C. Pratt, K. Josephson, N. McRedmond, K. Withycombe, J. Walsh, C. Matthews, D. Mahlangu, J. Krause, A. Schwyzer, R. Thejpal, R. Rapiti, N. Goga, Y. Coetzee, M. Stones, D. Mann, K. Butenas, S. Almasy, L. Blangero, J. Carless, M. Raja, R. Reed, E. |
| author_sort | Pandey, G. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Neutralizing antibodies (inhibitors) to replacement factor VIII (FVIII, either plasma derived or recombinant) impair the effective management of hemophilia A1. Individuals with hemophilia A due to major deletions of the FVIII gene (F8) lack antigenically cross-reactive material in their plasma (“CRM-negative”), and the prevalence of inhibitors in these individuals may be as high as 90%. Conversely, individuals with hemophilia A caused by F8 missense mutations are CRM-positive, and their overall prevalence of inhibitors is <10% (ref. 2). Individuals with the F8 intron 22 inversion (found in ~50% of individuals with severe hemophilia A) have been grouped with the former on the basis of their genetic defect and CRM-negative status. However, only ~20% of these individuals develop inhibitors3. Here we demonstrate that the levels of F8 mRNA and intracellular FVIII protein in B lymphoblastoid cells and liver biopsies from individuals with the intron 22 inversion are comparable to those in healthy controls. These results support the hypothesis that most individuals with the intron 22 inversion are tolerized to FVIII and thus do not develop inhibitors. Furthermore, we developed a new pharmacogenetic algorithm that permits the stratification of inhibitor risk for individuals and subpopulations by predicting the immunogenicity of replacement FVIII using, as input, the number of putative T cell epitopes in the infused protein and the competence of major histocompatibility complex class II molecules to present such epitopes. This algorithm showed statistically significant accuracy in predicting the presence of inhibitors in 25 unrelated individuals with the intron 22 inversion. |
| first_indexed | 2025-11-14T08:31:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-33146 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:31:17Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-331462018-03-29T09:08:37Z Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A Pandey, G. Yanover, C. Miller-Jenkins, L. Garfield, S. Cole, S. Curran, J. Moses, Eric Rydz, N. Simhadri, V. Kimchi-Sarfaty, C. Lillicrap, D. Viel, K. Przytycka, T. Pierce, G. Howard, T. Sauna, Z. Lusher, J. Chitlur, M. Ameri, A. Natarajan, K. Iyer, R. Thompson, A. Watts, R. Kempton, C. Kessler, C. Barrett, J. Martin, E. Key, N. Kruse-Jarres, R. Lessinger, C. Pratt, K. Josephson, N. McRedmond, K. Withycombe, J. Walsh, C. Matthews, D. Mahlangu, J. Krause, A. Schwyzer, R. Thejpal, R. Rapiti, N. Goga, Y. Coetzee, M. Stones, D. Mann, K. Butenas, S. Almasy, L. Blangero, J. Carless, M. Raja, R. Reed, E. Neutralizing antibodies (inhibitors) to replacement factor VIII (FVIII, either plasma derived or recombinant) impair the effective management of hemophilia A1. Individuals with hemophilia A due to major deletions of the FVIII gene (F8) lack antigenically cross-reactive material in their plasma (“CRM-negative”), and the prevalence of inhibitors in these individuals may be as high as 90%. Conversely, individuals with hemophilia A caused by F8 missense mutations are CRM-positive, and their overall prevalence of inhibitors is <10% (ref. 2). Individuals with the F8 intron 22 inversion (found in ~50% of individuals with severe hemophilia A) have been grouped with the former on the basis of their genetic defect and CRM-negative status. However, only ~20% of these individuals develop inhibitors3. Here we demonstrate that the levels of F8 mRNA and intracellular FVIII protein in B lymphoblastoid cells and liver biopsies from individuals with the intron 22 inversion are comparable to those in healthy controls. These results support the hypothesis that most individuals with the intron 22 inversion are tolerized to FVIII and thus do not develop inhibitors. Furthermore, we developed a new pharmacogenetic algorithm that permits the stratification of inhibitor risk for individuals and subpopulations by predicting the immunogenicity of replacement FVIII using, as input, the number of putative T cell epitopes in the infused protein and the competence of major histocompatibility complex class II molecules to present such epitopes. This algorithm showed statistically significant accuracy in predicting the presence of inhibitors in 25 unrelated individuals with the intron 22 inversion. 2013 Journal Article http://hdl.handle.net/20.500.11937/33146 10.1038/nm.3270 restricted |
| spellingShingle | Pandey, G. Yanover, C. Miller-Jenkins, L. Garfield, S. Cole, S. Curran, J. Moses, Eric Rydz, N. Simhadri, V. Kimchi-Sarfaty, C. Lillicrap, D. Viel, K. Przytycka, T. Pierce, G. Howard, T. Sauna, Z. Lusher, J. Chitlur, M. Ameri, A. Natarajan, K. Iyer, R. Thompson, A. Watts, R. Kempton, C. Kessler, C. Barrett, J. Martin, E. Key, N. Kruse-Jarres, R. Lessinger, C. Pratt, K. Josephson, N. McRedmond, K. Withycombe, J. Walsh, C. Matthews, D. Mahlangu, J. Krause, A. Schwyzer, R. Thejpal, R. Rapiti, N. Goga, Y. Coetzee, M. Stones, D. Mann, K. Butenas, S. Almasy, L. Blangero, J. Carless, M. Raja, R. Reed, E. Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A |
| title | Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A |
| title_full | Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A |
| title_fullStr | Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A |
| title_full_unstemmed | Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A |
| title_short | Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A |
| title_sort | endogenous factor viii synthesis from the intron 22-inverted f8 locus may modulate the immunogenicity of replacement therapy for hemophilia a |
| url | http://hdl.handle.net/20.500.11937/33146 |