Integrated microspectrometer for fluorescence based analysis in a microfluidic format
We have demonstrated a monolithic integrated arrayed waveguide grating (AWG) microspectrometer microfluidic platform capable of fluorescence spectroscopic analysis. The microspectrometer in this proof of concept study has a small (1 cm × 1 cm) footprint and 8 output channels centred on different wav...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
Royal Society of Chemistry
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/33054 |
| _version_ | 1848753838741258240 |
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| author | Hu, Z. Glidle, A. Ironside, Charlie Sorel, M. Strain, M. Cooper, J. Yin, H. |
| author_facet | Hu, Z. Glidle, A. Ironside, Charlie Sorel, M. Strain, M. Cooper, J. Yin, H. |
| author_sort | Hu, Z. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We have demonstrated a monolithic integrated arrayed waveguide grating (AWG) microspectrometer microfluidic platform capable of fluorescence spectroscopic analysis. The microspectrometer in this proof of concept study has a small (1 cm × 1 cm) footprint and 8 output channels centred on different wavelengths. We show that the signals from the output channels detected on a camera chip can be used to recreate the complete fluorescence spectrum of an analyte. By making fluorescence measurements of (i) mixed quantum dot solutions, (ii) an organic fluorophore (Cy5) and (iii) the propidium iodide (PI)-DNA assay, we illustrate the unique advantages of the AWG platform for simultaneous, quantitative multiplex detection and its capability to detect small spectroscopic shifts. Although the current system is designed for fluorescence spectroscopic analysis, in principle, it can be implemented for other types of analysis, such as Raman spectroscopy. Fabricated using established semiconductor industry methods, this miniaturised platform holds great potential to create a handheld, low cost biosensor with versatile detection capability. © The Royal Society of Chemistry 2012. |
| first_indexed | 2025-11-14T08:30:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-33054 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:30:53Z |
| publishDate | 2012 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-330542017-09-13T15:28:26Z Integrated microspectrometer for fluorescence based analysis in a microfluidic format Hu, Z. Glidle, A. Ironside, Charlie Sorel, M. Strain, M. Cooper, J. Yin, H. We have demonstrated a monolithic integrated arrayed waveguide grating (AWG) microspectrometer microfluidic platform capable of fluorescence spectroscopic analysis. The microspectrometer in this proof of concept study has a small (1 cm × 1 cm) footprint and 8 output channels centred on different wavelengths. We show that the signals from the output channels detected on a camera chip can be used to recreate the complete fluorescence spectrum of an analyte. By making fluorescence measurements of (i) mixed quantum dot solutions, (ii) an organic fluorophore (Cy5) and (iii) the propidium iodide (PI)-DNA assay, we illustrate the unique advantages of the AWG platform for simultaneous, quantitative multiplex detection and its capability to detect small spectroscopic shifts. Although the current system is designed for fluorescence spectroscopic analysis, in principle, it can be implemented for other types of analysis, such as Raman spectroscopy. Fabricated using established semiconductor industry methods, this miniaturised platform holds great potential to create a handheld, low cost biosensor with versatile detection capability. © The Royal Society of Chemistry 2012. 2012 Journal Article http://hdl.handle.net/20.500.11937/33054 10.1039/c2lc40169j Royal Society of Chemistry restricted |
| spellingShingle | Hu, Z. Glidle, A. Ironside, Charlie Sorel, M. Strain, M. Cooper, J. Yin, H. Integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| title | Integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| title_full | Integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| title_fullStr | Integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| title_full_unstemmed | Integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| title_short | Integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| title_sort | integrated microspectrometer for fluorescence based analysis in a microfluidic format |
| url | http://hdl.handle.net/20.500.11937/33054 |