A plasmid-transposon hybrid mutagenesis system effective in a broad range of enterobacteria
Random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. Here we develop a system facilitating random transposon mutagenesis in a range of different Gram-negative bacterial strains, including Pectobacterium atroseptic...
| Main Authors: | Monson, R., Smith, D., Matilla, M., Roberts, K., Richardson, E., Drew, A., Williamson, N., Ramsay, Joshua, Welch, M., Salmond, G. |
|---|---|
| Format: | Journal Article |
| Published: |
Frontiers Research Foundation
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/32913 |
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