Biopolymeric formulations for biocatalysis and biomedical applications
Three gel disks formulations prepared using chitosan (Chito) or polyethylenimine (PEI) followed by glutaraldehyde were prepared for biocatalysis and biomedical applications. The carriers have been used to immobilize lactase covalently and it was evaluated in terms of enzyme loading capacity and enzy...
| Main Authors: | , |
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| Format: | Journal Article |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/32783 |
| _version_ | 1848753759458426880 |
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| author | Elnashar, Magdy Kahil, T. |
| author_facet | Elnashar, Magdy Kahil, T. |
| author_sort | Elnashar, Magdy |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Three gel disks formulations prepared using chitosan (Chito) or polyethylenimine (PEI) followed by glutaraldehyde were prepared for biocatalysis and biomedical applications. The carriers have been used to immobilize lactase covalently and it was evaluated in terms of enzyme loading capacity and enzyme kinetics (km and Vmax). The Km of the Chito formulation was almost half that of the PEI formulations, which is favored in industries. On the other hand, the gel disks were evaluated in terms of their swelling kinetics and the gels' morphology using SEM. The mechanism of the three gels' swelling was also studied and it was found to be non-Fickian, where the mechanism of transport depends on both the diffusion and polymer relaxation, which are controlling the overall rate of water uptake. The Chito formulation was 2-5 folds and PEI formulations were 33-62 folds in terms of the swelling rate constant and the diffusion rate, respectively. These results were highly supported by the SEM. This study will help scientists to design the right polymer network for enzymes immobilization as well as control the surface area and the swelling power of the polymers for different applications such as drug delivery systems and tissue engineering. |
| first_indexed | 2025-11-14T08:29:37Z |
| format | Journal Article |
| id | curtin-20.500.11937-32783 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:29:37Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-327832017-09-13T15:25:36Z Biopolymeric formulations for biocatalysis and biomedical applications Elnashar, Magdy Kahil, T. Three gel disks formulations prepared using chitosan (Chito) or polyethylenimine (PEI) followed by glutaraldehyde were prepared for biocatalysis and biomedical applications. The carriers have been used to immobilize lactase covalently and it was evaluated in terms of enzyme loading capacity and enzyme kinetics (km and Vmax). The Km of the Chito formulation was almost half that of the PEI formulations, which is favored in industries. On the other hand, the gel disks were evaluated in terms of their swelling kinetics and the gels' morphology using SEM. The mechanism of the three gels' swelling was also studied and it was found to be non-Fickian, where the mechanism of transport depends on both the diffusion and polymer relaxation, which are controlling the overall rate of water uptake. The Chito formulation was 2-5 folds and PEI formulations were 33-62 folds in terms of the swelling rate constant and the diffusion rate, respectively. These results were highly supported by the SEM. This study will help scientists to design the right polymer network for enzymes immobilization as well as control the surface area and the swelling power of the polymers for different applications such as drug delivery systems and tissue engineering. 2014 Journal Article http://hdl.handle.net/20.500.11937/32783 10.1155/2014/418097 Hindawi Publishing Corporation fulltext |
| spellingShingle | Elnashar, Magdy Kahil, T. Biopolymeric formulations for biocatalysis and biomedical applications |
| title | Biopolymeric formulations for biocatalysis and biomedical applications |
| title_full | Biopolymeric formulations for biocatalysis and biomedical applications |
| title_fullStr | Biopolymeric formulations for biocatalysis and biomedical applications |
| title_full_unstemmed | Biopolymeric formulations for biocatalysis and biomedical applications |
| title_short | Biopolymeric formulations for biocatalysis and biomedical applications |
| title_sort | biopolymeric formulations for biocatalysis and biomedical applications |
| url | http://hdl.handle.net/20.500.11937/32783 |