Transformation of the nematode-trapping fungus Arthrobotrys oligospora
The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaC...
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Published: |
1999
|
| Online Access: | http://hdl.handle.net/20.500.11937/32750 |
| _version_ | 1848753749945745408 |
|---|---|
| author | Tunlid, A. Ahman, J. Oliver, Richard |
| author_facet | Tunlid, A. Ahman, J. Oliver, Richard |
| author_sort | Tunlid, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1–6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. |
| first_indexed | 2025-11-14T08:29:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-32750 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:29:28Z |
| publishDate | 1999 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-327502017-09-13T15:53:16Z Transformation of the nematode-trapping fungus Arthrobotrys oligospora Tunlid, A. Ahman, J. Oliver, Richard The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1–6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. 1999 Journal Article http://hdl.handle.net/20.500.11937/32750 10.1111/j.1574-6968.1999.tb13491.x restricted |
| spellingShingle | Tunlid, A. Ahman, J. Oliver, Richard Transformation of the nematode-trapping fungus Arthrobotrys oligospora |
| title | Transformation of the nematode-trapping fungus Arthrobotrys oligospora |
| title_full | Transformation of the nematode-trapping fungus Arthrobotrys oligospora |
| title_fullStr | Transformation of the nematode-trapping fungus Arthrobotrys oligospora |
| title_full_unstemmed | Transformation of the nematode-trapping fungus Arthrobotrys oligospora |
| title_short | Transformation of the nematode-trapping fungus Arthrobotrys oligospora |
| title_sort | transformation of the nematode-trapping fungus arthrobotrys oligospora |
| url | http://hdl.handle.net/20.500.11937/32750 |