Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture
We investigated the short-term effect of LIPUS on human dentin-pulp complex in vitro. We collected sixty-three premolars from patients who needed the extraction. The premolars were sectioned transversely into 600-µm-thick slices, and then divided into five groups according to LIPUS application time...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Elsevier Inc.
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/32709 |
| _version_ | 1848753738929405952 |
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| author | Al-Daghreer, S. Doschak, Michael Sloan, A. Major, P. Heo, G. Scurtescu, C. Tsui, Y. El-Bialy, T. |
| author_facet | Al-Daghreer, S. Doschak, Michael Sloan, A. Major, P. Heo, G. Scurtescu, C. Tsui, Y. El-Bialy, T. |
| author_sort | Al-Daghreer, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We investigated the short-term effect of LIPUS on human dentin-pulp complex in vitro. We collected sixty-three premolars from patients who needed the extraction. The premolars were sectioned transversely into 600-µm-thick slices, and then divided into five groups according to LIPUS application time (control, 5, 10, 15 and 20 min). LIPUS transducer produced an incident intensity of 30 mW/cm2. After 24 h, tissue was harvested for histomorphometrical analysis and RT-PCR (Genes of interest: Collagen I, DMP1, DSPP, TGF ß1, RANKL and OPG). Histomorphometric analysis showed no significant difference among the five groups in the odontoblast count and predentin thickness. RT-PCR demonstrated no expression of TGF ß1, low amounts of DSPP, a twofold increase in collagen I expression in the 5- and 10-minute LIPUS groups and a threefold increase in DMP1 expression in the 10-minute LIPUS group. LIPUS application was stimulatory to the dentin-pulp complex in vitro and increased the expression of collagen I and DMP1. |
| first_indexed | 2025-11-14T08:29:18Z |
| format | Journal Article |
| id | curtin-20.500.11937-32709 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:29:18Z |
| publishDate | 2013 |
| publisher | Elsevier Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-327092017-09-13T15:27:37Z Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture Al-Daghreer, S. Doschak, Michael Sloan, A. Major, P. Heo, G. Scurtescu, C. Tsui, Y. El-Bialy, T. DMP1 odontoblast LIPUS predentin dentin-pulp complex We investigated the short-term effect of LIPUS on human dentin-pulp complex in vitro. We collected sixty-three premolars from patients who needed the extraction. The premolars were sectioned transversely into 600-µm-thick slices, and then divided into five groups according to LIPUS application time (control, 5, 10, 15 and 20 min). LIPUS transducer produced an incident intensity of 30 mW/cm2. After 24 h, tissue was harvested for histomorphometrical analysis and RT-PCR (Genes of interest: Collagen I, DMP1, DSPP, TGF ß1, RANKL and OPG). Histomorphometric analysis showed no significant difference among the five groups in the odontoblast count and predentin thickness. RT-PCR demonstrated no expression of TGF ß1, low amounts of DSPP, a twofold increase in collagen I expression in the 5- and 10-minute LIPUS groups and a threefold increase in DMP1 expression in the 10-minute LIPUS group. LIPUS application was stimulatory to the dentin-pulp complex in vitro and increased the expression of collagen I and DMP1. 2013 Journal Article http://hdl.handle.net/20.500.11937/32709 10.1016/j.ultrasmedbio.2012.12.005 Elsevier Inc. restricted |
| spellingShingle | DMP1 odontoblast LIPUS predentin dentin-pulp complex Al-Daghreer, S. Doschak, Michael Sloan, A. Major, P. Heo, G. Scurtescu, C. Tsui, Y. El-Bialy, T. Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture |
| title | Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture |
| title_full | Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture |
| title_fullStr | Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture |
| title_full_unstemmed | Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture |
| title_short | Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture |
| title_sort | short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-d tooth culture |
| topic | DMP1 odontoblast LIPUS predentin dentin-pulp complex |
| url | http://hdl.handle.net/20.500.11937/32709 |