Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS

Cyclodextrin GC stationary phases, well documented for their superior isomeric separation capabilities, have been investigated in the separation of crocetane (2,6,11,15-tetramethylhexadecane) and phytane (2,6,10,14-tetramethylhexadecane). Although crocetane has been attributed to methane oxidizing a...

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Main Authors: Barber, Cindy, Bastow, Trevor, Grice, Kliti, Alexander, Robert, Kagi, Robert
Format: Journal Article
Published: Pergamon-Elsevier 2001
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/32646
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author Barber, Cindy
Bastow, Trevor
Grice, Kliti
Alexander, Robert
Kagi, Robert
author_facet Barber, Cindy
Bastow, Trevor
Grice, Kliti
Alexander, Robert
Kagi, Robert
author_sort Barber, Cindy
building Curtin Institutional Repository
collection Online Access
description Cyclodextrin GC stationary phases, well documented for their superior isomeric separation capabilities, have been investigated in the separation of crocetane (2,6,11,15-tetramethylhexadecane) and phytane (2,6,10,14-tetramethylhexadecane). Although crocetane has been attributed to methane oxidizing archaea, its general occurrence in crude oils and sediments is yet to be fully established due to difficulties associated with the GC?MS analysis since crocetane co-elutes with phytane when chromatographed on most liquid phase coated capillary columns. A method is described for the routine GC-MS analysis of crocetane and phytane in crude oils and sediments, using cyclodextrinstationary phases. Numerous derivatised cyclodextrin columns were investigated. A 50 m permethyl-b-cyclodextrin was found to give the best resolution (R=0.8 using hydrogen as the carrier gas and R=0.7 using helium).
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institution Curtin University Malaysia
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publishDate 2001
publisher Pergamon-Elsevier
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spelling curtin-20.500.11937-326462017-09-13T15:55:19Z Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS Barber, Cindy Bastow, Trevor Grice, Kliti Alexander, Robert Kagi, Robert Phytane Cyclodextrin stationary phases Crocetane Cyclodextrin GC stationary phases, well documented for their superior isomeric separation capabilities, have been investigated in the separation of crocetane (2,6,11,15-tetramethylhexadecane) and phytane (2,6,10,14-tetramethylhexadecane). Although crocetane has been attributed to methane oxidizing archaea, its general occurrence in crude oils and sediments is yet to be fully established due to difficulties associated with the GC?MS analysis since crocetane co-elutes with phytane when chromatographed on most liquid phase coated capillary columns. A method is described for the routine GC-MS analysis of crocetane and phytane in crude oils and sediments, using cyclodextrinstationary phases. Numerous derivatised cyclodextrin columns were investigated. A 50 m permethyl-b-cyclodextrin was found to give the best resolution (R=0.8 using hydrogen as the carrier gas and R=0.7 using helium). 2001 Journal Article http://hdl.handle.net/20.500.11937/32646 10.1016/S0146-6380(01)00031-6 Pergamon-Elsevier restricted
spellingShingle Phytane
Cyclodextrin stationary phases
Crocetane
Barber, Cindy
Bastow, Trevor
Grice, Kliti
Alexander, Robert
Kagi, Robert
Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS
title Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS
title_full Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS
title_fullStr Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS
title_full_unstemmed Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS
title_short Analysis of crocetane in crude oils and sediments: novel stationary phases for use in GC-MS
title_sort analysis of crocetane in crude oils and sediments: novel stationary phases for use in gc-ms
topic Phytane
Cyclodextrin stationary phases
Crocetane
url http://hdl.handle.net/20.500.11937/32646