Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study
Microencapsulated pancreatic ß-cells have been studied as an ideal system for delivering insulin. However, the clinical applications have not been achieved. We tried multiple excipients/ratios and various methods for producing suitable microcapsules, but success remains limited. A recent study used...
| Main Authors: | , , |
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| Format: | Journal Article |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/32621 |
| _version_ | 1848753713519263744 |
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| author | Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani |
| author_facet | Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani |
| author_sort | Mooranian, Armin |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Microencapsulated pancreatic ß-cells have been studied as an ideal system for delivering insulin. However, the clinical applications have not been achieved. We tried multiple excipients/ratios and various methods for producing suitable microcapsules, but success remains limited. A recent study used the tertiary bile acid, taurocholic acid (TCA), to improve the rectal delivery of insulin while another study used TCA to improve the oral delivery of heparin. Thus, in this study, we used TCA in different formulations, using a new microencapsulating method, to study its suitability for ß-cell microencapsulation. Using a methodology at set temperature of 37°C, amplitude of 1.5 and a total gelation time of 21 minutes, ten different formulations were examined (with or without TCA) and all formulations showed similar cell viability, which was enhanced by TCA addition. This suggests potential benefits of our microencapsulating parameters and TCA in ß-cells microencapsulation. |
| first_indexed | 2025-11-14T08:28:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-32621 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:28:53Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-326212017-09-13T15:25:11Z Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani Microencapsulated pancreatic ß-cells have been studied as an ideal system for delivering insulin. However, the clinical applications have not been achieved. We tried multiple excipients/ratios and various methods for producing suitable microcapsules, but success remains limited. A recent study used the tertiary bile acid, taurocholic acid (TCA), to improve the rectal delivery of insulin while another study used TCA to improve the oral delivery of heparin. Thus, in this study, we used TCA in different formulations, using a new microencapsulating method, to study its suitability for ß-cell microencapsulation. Using a methodology at set temperature of 37°C, amplitude of 1.5 and a total gelation time of 21 minutes, ten different formulations were examined (with or without TCA) and all formulations showed similar cell viability, which was enhanced by TCA addition. This suggests potential benefits of our microencapsulating parameters and TCA in ß-cells microencapsulation. 2016 Journal Article http://hdl.handle.net/20.500.11937/32621 10.1504/IJNBM.2016.079684 restricted |
| spellingShingle | Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study |
| title | Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study |
| title_full | Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study |
| title_fullStr | Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study |
| title_full_unstemmed | Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study |
| title_short | Viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: An ex vivo study |
| title_sort | viability and topographical analysis of microencapsulated ß-cells exposed to a biotransformed tertiary bile acid: an ex vivo study |
| url | http://hdl.handle.net/20.500.11937/32621 |