Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration
The migration of cells is fundamental to a number of physiological/pathological processes, ranging from embryonic development, tissue regeneration to cancer metastasis. Current research on cell migration is largely based on simplified in vitro models that assume a homogeneous microenvironment and ov...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Springer New York LLC
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/32537 |
| _version_ | 1848753691125874688 |
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| author | Sun, W. Kurniawan, N. Kumar, Alan Prem Rajagopalan, R. Lim, C. |
| author_facet | Sun, W. Kurniawan, N. Kumar, Alan Prem Rajagopalan, R. Lim, C. |
| author_sort | Sun, W. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The migration of cells is fundamental to a number of physiological/pathological processes, ranging from embryonic development, tissue regeneration to cancer metastasis. Current research on cell migration is largely based on simplified in vitro models that assume a homogeneous microenvironment and overlook the modification of extracellular matrix (ECM) by the cells. To address this shortcoming, we developed a nested three-dimensional (3D) collagen hydrogel model mimicking the connective tissue confronted by highly malignant breast cancer cells, MDA-MB-231. Strikingly, our findings revealed two distinct cell migration patterns: a rapid and directionally persistent collective migration of the leader cells and a more randomized migration in the regions that have previously been significantly modified by cells. The cell-induced modifications, which typically include clustering and alignment of fibers, effectively segmented the matrix into smaller sub-regions. Our results suggest that in an elastic 3D matrix, the presence of adjacent cells that have modified the matrix may in fact become physical hurdle to a migrating cell. Furthermore, our study emphasizes the need for a micromechanical understanding in the context of cancer invasion that allows for cell-induced modification of ECM and a heterogeneous cell migration. |
| first_indexed | 2025-11-14T08:28:32Z |
| format | Journal Article |
| id | curtin-20.500.11937-32537 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:28:32Z |
| publishDate | 2014 |
| publisher | Springer New York LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-325372018-03-29T09:08:13Z Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration Sun, W. Kurniawan, N. Kumar, Alan Prem Rajagopalan, R. Lim, C. Cell contraction 3D extracellular matrix Collective cell migration Cancer invasion Matrix remodeling The migration of cells is fundamental to a number of physiological/pathological processes, ranging from embryonic development, tissue regeneration to cancer metastasis. Current research on cell migration is largely based on simplified in vitro models that assume a homogeneous microenvironment and overlook the modification of extracellular matrix (ECM) by the cells. To address this shortcoming, we developed a nested three-dimensional (3D) collagen hydrogel model mimicking the connective tissue confronted by highly malignant breast cancer cells, MDA-MB-231. Strikingly, our findings revealed two distinct cell migration patterns: a rapid and directionally persistent collective migration of the leader cells and a more randomized migration in the regions that have previously been significantly modified by cells. The cell-induced modifications, which typically include clustering and alignment of fibers, effectively segmented the matrix into smaller sub-regions. Our results suggest that in an elastic 3D matrix, the presence of adjacent cells that have modified the matrix may in fact become physical hurdle to a migrating cell. Furthermore, our study emphasizes the need for a micromechanical understanding in the context of cancer invasion that allows for cell-induced modification of ECM and a heterogeneous cell migration. 2014 Journal Article http://hdl.handle.net/20.500.11937/32537 10.1007/s12195-014-0324-0 Springer New York LLC restricted |
| spellingShingle | Cell contraction 3D extracellular matrix Collective cell migration Cancer invasion Matrix remodeling Sun, W. Kurniawan, N. Kumar, Alan Prem Rajagopalan, R. Lim, C. Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration |
| title | Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration |
| title_full | Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration |
| title_fullStr | Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration |
| title_full_unstemmed | Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration |
| title_short | Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration |
| title_sort | effects of migrating cell-induced matrix reorganization on 3d cancer cell migration |
| topic | Cell contraction 3D extracellular matrix Collective cell migration Cancer invasion Matrix remodeling |
| url | http://hdl.handle.net/20.500.11937/32537 |