Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production
Phenotypic mutants of Sporosarcina pasteurii (previously known as Bacillus pasteurii) (MTCC 1761) were developed by UV irradiation to test their ability to enhance urease activity and calcite production. Among the mutants, Bp M-3 was found to be more efficient compared to other mutants and wild-type...
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| Format: | Journal Article |
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Han'gug Mi'saengmul Saengmyeong Gong Haghoe
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/32512 |
| _version_ | 1848753684041695232 |
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| author | Achal, V. Mukherjee, Abhijit Basu, P. Reddy, M. |
| author_facet | Achal, V. Mukherjee, Abhijit Basu, P. Reddy, M. |
| author_sort | Achal, V. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Phenotypic mutants of Sporosarcina pasteurii (previously known as Bacillus pasteurii) (MTCC 1761) were developed by UV irradiation to test their ability to enhance urease activity and calcite production. Among the mutants, Bp M-3 was found to be more efficient compared to other mutants and wild-type strain. It produced the highest urease activity and calcite production compared to other isolates. The production of extracellular polymeric substances and biofilm was also higher in this mutant than other isolates. Microbial sand plugging results showed the highest calcite precipitation by Bp M-3 mutant. Scanning electron micrography, energy-dispersive X-ray and X-ray diffraction analyses evidenced the direct involvement of bacteria in CaCO 3 precipitation. This study suggests that calcite production by the mutant through biomineralization processes is highly effective and may provide a useful strategy as a sealing agent for filling the gaps or cracks and fissures in any construction structures. |
| first_indexed | 2025-11-14T08:28:25Z |
| format | Journal Article |
| id | curtin-20.500.11937-32512 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:28:25Z |
| publishDate | 2009 |
| publisher | Han'gug Mi'saengmul Saengmyeong Gong Haghoe |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-325122017-09-13T15:24:46Z Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production Achal, V. Mukherjee, Abhijit Basu, P. Reddy, M. Extracellular polymeric substances Calcite precipitation Biofilm Sporosarcina pasteurii (Bacillus pasteurii) Urease UV-induced mutants Phenotypic mutants of Sporosarcina pasteurii (previously known as Bacillus pasteurii) (MTCC 1761) were developed by UV irradiation to test their ability to enhance urease activity and calcite production. Among the mutants, Bp M-3 was found to be more efficient compared to other mutants and wild-type strain. It produced the highest urease activity and calcite production compared to other isolates. The production of extracellular polymeric substances and biofilm was also higher in this mutant than other isolates. Microbial sand plugging results showed the highest calcite precipitation by Bp M-3 mutant. Scanning electron micrography, energy-dispersive X-ray and X-ray diffraction analyses evidenced the direct involvement of bacteria in CaCO 3 precipitation. This study suggests that calcite production by the mutant through biomineralization processes is highly effective and may provide a useful strategy as a sealing agent for filling the gaps or cracks and fissures in any construction structures. 2009 Journal Article http://hdl.handle.net/20.500.11937/32512 10.1007/s10295-009-0578-z Han'gug Mi'saengmul Saengmyeong Gong Haghoe restricted |
| spellingShingle | Extracellular polymeric substances Calcite precipitation Biofilm Sporosarcina pasteurii (Bacillus pasteurii) Urease UV-induced mutants Achal, V. Mukherjee, Abhijit Basu, P. Reddy, M. Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production |
| title | Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production |
| title_full | Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production |
| title_fullStr | Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production |
| title_full_unstemmed | Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production |
| title_short | Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production |
| title_sort | strain improvement of sporosarcina pasteurii for enhanced urease and calcite production |
| topic | Extracellular polymeric substances Calcite precipitation Biofilm Sporosarcina pasteurii (Bacillus pasteurii) Urease UV-induced mutants |
| url | http://hdl.handle.net/20.500.11937/32512 |