Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate

In this study we investigate the electrochemical synthesis and characterisation of a nanometre scale porous anodic aluminium oxide (AAO) membranes with a mean pore diameter of 100 nm. The membranes exhibit interesting properties such as controllable pore diameters, periodicity and density distributi...

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Main Authors: Poinern, Gerrard, Le, Xuan, Hager, Marilyn, Becker, Thomas, Fawcett, Derek
Format: Journal Article
Published: Scientific & Academic Publishing 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/32349
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author Poinern, Gerrard
Le, Xuan
Hager, Marilyn
Becker, Thomas
Fawcett, Derek
author_facet Poinern, Gerrard
Le, Xuan
Hager, Marilyn
Becker, Thomas
Fawcett, Derek
author_sort Poinern, Gerrard
building Curtin Institutional Repository
collection Online Access
description In this study we investigate the electrochemical synthesis and characterisation of a nanometre scale porous anodic aluminium oxide (AAO) membranes with a mean pore diameter of 100 nm. The membranes exhibit interesting properties such as controllable pore diameters, periodicity and density distribution. These properties can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The surface features of the nanometre scale membrane such as pore density, pore diameter and inter-pore distance were quantified using field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM). A preliminary biological evaluation of the membranes was carried out to determine cell adhesion and morphology using the Cercopithecus aethiops[African green monkey – (Vero)] kidney epithelial cell line. Optical microscopy, FESEM and AFM investigations revealed the presence of focal adhesion sites over the surface of the porous membranes. The positive outcomes of the study, indicates that AAO membranes can be used as a viable tissue scaffold for potential tissue engineering applications in the future.
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institution Curtin University Malaysia
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publishDate 2013
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spelling curtin-20.500.11937-323492017-09-13T15:23:29Z Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate Poinern, Gerrard Le, Xuan Hager, Marilyn Becker, Thomas Fawcett, Derek Tissue Scaffold Regenerative Tissues Nano-porous Anodic Aluminium Oxide Cell Substrate In this study we investigate the electrochemical synthesis and characterisation of a nanometre scale porous anodic aluminium oxide (AAO) membranes with a mean pore diameter of 100 nm. The membranes exhibit interesting properties such as controllable pore diameters, periodicity and density distribution. These properties can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The surface features of the nanometre scale membrane such as pore density, pore diameter and inter-pore distance were quantified using field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM). A preliminary biological evaluation of the membranes was carried out to determine cell adhesion and morphology using the Cercopithecus aethiops[African green monkey – (Vero)] kidney epithelial cell line. Optical microscopy, FESEM and AFM investigations revealed the presence of focal adhesion sites over the surface of the porous membranes. The positive outcomes of the study, indicates that AAO membranes can be used as a viable tissue scaffold for potential tissue engineering applications in the future. 2013 Journal Article http://hdl.handle.net/20.500.11937/32349 10.5923/j.ajbe.20130306.01 Scientific & Academic Publishing restricted
spellingShingle Tissue Scaffold
Regenerative Tissues
Nano-porous Anodic Aluminium Oxide
Cell Substrate
Poinern, Gerrard
Le, Xuan
Hager, Marilyn
Becker, Thomas
Fawcett, Derek
Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate
title Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate
title_full Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate
title_fullStr Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate
title_full_unstemmed Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate
title_short Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate
title_sort electrochemical synthesis, characterisation, and preliminary biological evaluation of an anodic aluminium oxide membrane with a pore size of 100 nanometres for a potential cell culture substrate
topic Tissue Scaffold
Regenerative Tissues
Nano-porous Anodic Aluminium Oxide
Cell Substrate
url http://hdl.handle.net/20.500.11937/32349