Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113
Pseudomonas fluorescens F113 is a plant growth promoting rhizobacterium (PGPR) isolated from the sugar beet rhizosphere. The recent annotation of the F113 genome sequence has revealed that this strain encodes a wide array of secretion systems, including two complete type three secretion systems (T3S...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Wiley-Blackwell Publishing Ltd.
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/31727 |
| _version_ | 1848753462686253056 |
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| author | Barret, Matthieu Egan, Frank Moynihan, Jennifer Morrissey, John Lesouhaitier, Olivier O'Gara, Fergal |
| author_facet | Barret, Matthieu Egan, Frank Moynihan, Jennifer Morrissey, John Lesouhaitier, Olivier O'Gara, Fergal |
| author_sort | Barret, Matthieu |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Pseudomonas fluorescens F113 is a plant growth promoting rhizobacterium (PGPR) isolated from the sugar beet rhizosphere. The recent annotation of the F113 genome sequence has revealed that this strain encodes a wide array of secretion systems, including two complete type three secretion systems (T3SSs) belonging to the Hrp1 and SPI-1 families. While Hrp1 T3SSs are frequently encoded in other P. fluorescens strains, the presence of a SPI-1 T3SS in a plant beneficial bacterial strain was unexpected. In this work, the genetic organization and expression of these two T3SS loci have been analysed by a combination of transcriptional reporter fusions and transcriptome analyses. Overexpression of two transcriptional activators has shown a number of genes encoding putative T3 effectors. In addition, the influence of these two T3SSs during the interaction of P. fluorescens F113 with some bacterial predators was also assessed. Our data revealed that the transcriptional activator hilA is induced by amoeba and that the SPI-1 T3SS could potentially be involved in resistance to amoeboid grazing. |
| first_indexed | 2025-11-14T08:24:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-31727 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:24:54Z |
| publishDate | 2013 |
| publisher | Wiley-Blackwell Publishing Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-317272017-09-13T15:19:35Z Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 Barret, Matthieu Egan, Frank Moynihan, Jennifer Morrissey, John Lesouhaitier, Olivier O'Gara, Fergal Pseudomonas fluorescens F113 is a plant growth promoting rhizobacterium (PGPR) isolated from the sugar beet rhizosphere. The recent annotation of the F113 genome sequence has revealed that this strain encodes a wide array of secretion systems, including two complete type three secretion systems (T3SSs) belonging to the Hrp1 and SPI-1 families. While Hrp1 T3SSs are frequently encoded in other P. fluorescens strains, the presence of a SPI-1 T3SS in a plant beneficial bacterial strain was unexpected. In this work, the genetic organization and expression of these two T3SS loci have been analysed by a combination of transcriptional reporter fusions and transcriptome analyses. Overexpression of two transcriptional activators has shown a number of genes encoding putative T3 effectors. In addition, the influence of these two T3SSs during the interaction of P. fluorescens F113 with some bacterial predators was also assessed. Our data revealed that the transcriptional activator hilA is induced by amoeba and that the SPI-1 T3SS could potentially be involved in resistance to amoeboid grazing. 2013 Journal Article http://hdl.handle.net/20.500.11937/31727 10.1111/1758-2229.12039 Wiley-Blackwell Publishing Ltd. restricted |
| spellingShingle | Barret, Matthieu Egan, Frank Moynihan, Jennifer Morrissey, John Lesouhaitier, Olivier O'Gara, Fergal Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 |
| title | Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 |
| title_full | Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 |
| title_fullStr | Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 |
| title_full_unstemmed | Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 |
| title_short | Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113 |
| title_sort | characterization of the spi-1 and rsp type three secretion systems in pseudomonas fluorescens f113 |
| url | http://hdl.handle.net/20.500.11937/31727 |