Direct electrochemistry of cytochrome c at modified Si(100) electrodes
This paper demonstrates the direct electron transfer between the heme moiety of horse hearth cytochrome c and a pyridinyl group on self-assembled-monolayer-modified Si(100) electrodes. Self-assembled monolayers (SAMs) containing the putative receptor ligand were prepared by a step-wise procedure usi...
| Main Authors: | , |
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| Format: | Journal Article |
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Wiley - V C H Verlag GmbH & Co. KGaA
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/31721 |
| _version_ | 1848753460979171328 |
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| author | Ciampi, Simone Gooding, J. |
| author_facet | Ciampi, Simone Gooding, J. |
| author_sort | Ciampi, Simone |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This paper demonstrates the direct electron transfer between the heme moiety of horse hearth cytochrome c and a pyridinyl group on self-assembled-monolayer-modified Si(100) electrodes. Self-assembled monolayers (SAMs) containing the putative receptor ligand were prepared by a step-wise procedure using "click" reactions of acetylene-terminated alkyl monolayers and isonicotinic acid azide derivatives. Unoxidized Si(100) electrodes, possessing either isonicotinate or isonicotinamide receptor ligands, were characterized using X-ray photoelectron spectroscopy, contact-angle goniometry, cyclic voltammetry, and electrochemical impedance spectroscopy. The ability of isonicotinic acid terminated layers to coordinatively bind the redox center of cytochrome c was found to be restricted to pyridinyl assemblies with a paraester linkage present. The protocol detailed here offers an experimentally simple modular approach to producing chemically well-defined SAMs on silicon surfaces for direct electrochemistry of a well-studied model redox protein. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. |
| first_indexed | 2025-11-14T08:24:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-31721 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:24:53Z |
| publishDate | 2010 |
| publisher | Wiley - V C H Verlag GmbH & Co. KGaA |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-317212017-09-13T15:20:01Z Direct electrochemistry of cytochrome c at modified Si(100) electrodes Ciampi, Simone Gooding, J. This paper demonstrates the direct electron transfer between the heme moiety of horse hearth cytochrome c and a pyridinyl group on self-assembled-monolayer-modified Si(100) electrodes. Self-assembled monolayers (SAMs) containing the putative receptor ligand were prepared by a step-wise procedure using "click" reactions of acetylene-terminated alkyl monolayers and isonicotinic acid azide derivatives. Unoxidized Si(100) electrodes, possessing either isonicotinate or isonicotinamide receptor ligands, were characterized using X-ray photoelectron spectroscopy, contact-angle goniometry, cyclic voltammetry, and electrochemical impedance spectroscopy. The ability of isonicotinic acid terminated layers to coordinatively bind the redox center of cytochrome c was found to be restricted to pyridinyl assemblies with a paraester linkage present. The protocol detailed here offers an experimentally simple modular approach to producing chemically well-defined SAMs on silicon surfaces for direct electrochemistry of a well-studied model redox protein. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. 2010 Journal Article http://hdl.handle.net/20.500.11937/31721 10.1002/chem.200903316 Wiley - V C H Verlag GmbH & Co. KGaA restricted |
| spellingShingle | Ciampi, Simone Gooding, J. Direct electrochemistry of cytochrome c at modified Si(100) electrodes |
| title | Direct electrochemistry of cytochrome c at modified Si(100) electrodes |
| title_full | Direct electrochemistry of cytochrome c at modified Si(100) electrodes |
| title_fullStr | Direct electrochemistry of cytochrome c at modified Si(100) electrodes |
| title_full_unstemmed | Direct electrochemistry of cytochrome c at modified Si(100) electrodes |
| title_short | Direct electrochemistry of cytochrome c at modified Si(100) electrodes |
| title_sort | direct electrochemistry of cytochrome c at modified si(100) electrodes |
| url | http://hdl.handle.net/20.500.11937/31721 |