Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia

The Warehouse beetle, Trogoderma variabile (Coleoptera: Dermestidae), is an internationally significant invasive pest of packed goods and stored grain. When it was first documented in Australia at Griffith, New South Wales, in 1977, an eradication campaign was initiated. After several years and cons...

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Main Authors: Castalanelli, Mark, Mikac, K., Baker, A., Munyard, Kylie, Grimm, M., Groth, David
Format: Journal Article
Published: Cambridge University Press 2010 2011
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/31587
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author Castalanelli, Mark
Mikac, K.
Baker, A.
Munyard, Kylie
Grimm, M.
Groth, David
author_facet Castalanelli, Mark
Mikac, K.
Baker, A.
Munyard, Kylie
Grimm, M.
Groth, David
author_sort Castalanelli, Mark
building Curtin Institutional Repository
collection Online Access
description The Warehouse beetle, Trogoderma variabile (Coleoptera: Dermestidae), is an internationally significant invasive pest of packed goods and stored grain. When it was first documented in Australia at Griffith, New South Wales, in 1977, an eradication campaign was initiated. After several years and considerable effort, the eradication campaign was abandoned. To monitor the presence and spread of T. variabile, surveys were carried out by government agencies in 1992 and 2002. When survey data was compared, it was concluded that the distribution of morphologically identified T. variabile had doubled in most Australian states. Here, we used samples from the 2002 survey to conduct a phylogenetic study using partial sequences of mitochondrial genes Cytochrome oxidase I and Cytochrome B, and the nuclear gene 18S, to examine the distribution and dispersal of T. variabile and detect the presence of misidentified species. Based on our molecular results, we show that only 47% of the samples analysed were T. variabile, and the remaining were a mixture of six putative species. In addition, T. variabile was found in only 78% of the trapping sites. We discuss the importance of correct diagnosis in relation to the eradication campaign.
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spelling curtin-20.500.11937-315872017-10-02T02:28:16Z Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia Castalanelli, Mark Mikac, K. Baker, A. Munyard, Kylie Grimm, M. Groth, David Warehouse beetle eradication cryptic invasive correct diagnosis molecular population The Warehouse beetle, Trogoderma variabile (Coleoptera: Dermestidae), is an internationally significant invasive pest of packed goods and stored grain. When it was first documented in Australia at Griffith, New South Wales, in 1977, an eradication campaign was initiated. After several years and considerable effort, the eradication campaign was abandoned. To monitor the presence and spread of T. variabile, surveys were carried out by government agencies in 1992 and 2002. When survey data was compared, it was concluded that the distribution of morphologically identified T. variabile had doubled in most Australian states. Here, we used samples from the 2002 survey to conduct a phylogenetic study using partial sequences of mitochondrial genes Cytochrome oxidase I and Cytochrome B, and the nuclear gene 18S, to examine the distribution and dispersal of T. variabile and detect the presence of misidentified species. Based on our molecular results, we show that only 47% of the samples analysed were T. variabile, and the remaining were a mixture of six putative species. In addition, T. variabile was found in only 78% of the trapping sites. We discuss the importance of correct diagnosis in relation to the eradication campaign. 2011 Journal Article http://hdl.handle.net/20.500.11937/31587 10.1017/S0007485310000544 Cambridge University Press 2010 fulltext
spellingShingle Warehouse beetle
eradication
cryptic
invasive
correct diagnosis
molecular
population
Castalanelli, Mark
Mikac, K.
Baker, A.
Munyard, Kylie
Grimm, M.
Groth, David
Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia
title Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia
title_full Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia
title_fullStr Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia
title_full_unstemmed Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia
title_short Multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the Trogoderma variabile (Coleoptera: Dermestidae) trapping program in Australia
title_sort multiple incursions and putative species revealed using a mitochondrial and nuclear phylogenetic approach to the trogoderma variabile (coleoptera: dermestidae) trapping program in australia
topic Warehouse beetle
eradication
cryptic
invasive
correct diagnosis
molecular
population
url http://hdl.handle.net/20.500.11937/31587