Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry
© 2015 Elsevier B.V.Multi-beam wide-field imaging using synchrotron mid-infrared light sources coupled with focal plane array detectors has provided a major breakthrough to the field of bio-spectroscopic imaging. The ability to collect sub-cellular molecular images in minutes has opened the door to...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/31585 |
| _version_ | 1848753420953976832 |
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| author | Hackett, Mark Caine, S. Liu, X. May, T. Borondics, F. |
| author_facet | Hackett, Mark Caine, S. Liu, X. May, T. Borondics, F. |
| author_sort | Hackett, Mark |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2015 Elsevier B.V.Multi-beam wide-field imaging using synchrotron mid-infrared light sources coupled with focal plane array detectors has provided a major breakthrough to the field of bio-spectroscopic imaging. The ability to collect sub-cellular molecular images in minutes has opened the door to a new era of biochemical studies. Although a multi-beam approach is the superior method to this form of imaging, it requires a specialized set of beamline optics, which may not be compatible with existing mid-infrared microscopy beamlines, or research programs/applications currently in place (some of which do not require an imaging component). In this investigation we demonstrate that a single-beam approach can be utilized in a similar manner to multi-beam imaging, to collect sub-cellular biochemical images of brain neurons in a rapid time frame, without extensive modification of an existing beamline configuration. This study uses an applied example, imaging the same neuron in situ within a brain tissue section, with both synchrotron and thermal sources. The results highlight the advantage of improved spatial resolution/image quality and spectral quality (signal to noise ratio) that is obtained when a high magnification and high numerical aperture objective (52×, 0.65) is coupled to a synchrotron mid-infrared lightsource with a focal plane array detector. The approach we report may prove to be particularly appealing to numerous existing mid-infrared beamlines, allowing straightforward integration of sub-cellular biochemical imaging with existing non-imaging research applications. |
| first_indexed | 2025-11-14T08:24:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-31585 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:24:14Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-315852017-09-13T15:21:25Z Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry Hackett, Mark Caine, S. Liu, X. May, T. Borondics, F. © 2015 Elsevier B.V.Multi-beam wide-field imaging using synchrotron mid-infrared light sources coupled with focal plane array detectors has provided a major breakthrough to the field of bio-spectroscopic imaging. The ability to collect sub-cellular molecular images in minutes has opened the door to a new era of biochemical studies. Although a multi-beam approach is the superior method to this form of imaging, it requires a specialized set of beamline optics, which may not be compatible with existing mid-infrared microscopy beamlines, or research programs/applications currently in place (some of which do not require an imaging component). In this investigation we demonstrate that a single-beam approach can be utilized in a similar manner to multi-beam imaging, to collect sub-cellular biochemical images of brain neurons in a rapid time frame, without extensive modification of an existing beamline configuration. This study uses an applied example, imaging the same neuron in situ within a brain tissue section, with both synchrotron and thermal sources. The results highlight the advantage of improved spatial resolution/image quality and spectral quality (signal to noise ratio) that is obtained when a high magnification and high numerical aperture objective (52×, 0.65) is coupled to a synchrotron mid-infrared lightsource with a focal plane array detector. The approach we report may prove to be particularly appealing to numerous existing mid-infrared beamlines, allowing straightforward integration of sub-cellular biochemical imaging with existing non-imaging research applications. 2015 Journal Article http://hdl.handle.net/20.500.11937/31585 10.1016/j.vibspec.2014.12.004 Elsevier restricted |
| spellingShingle | Hackett, Mark Caine, S. Liu, X. May, T. Borondics, F. Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| title | Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| title_full | Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| title_fullStr | Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| title_full_unstemmed | Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| title_short | Development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| title_sort | development of single-beam wide-field infrared imaging to study sub-cellular neuron biochemistry |
| url | http://hdl.handle.net/20.500.11937/31585 |