Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae)
Premise of the study: Microsatellite markers were developed for the rare sedge Lepidosperma bungalbin (Cyperaceae) to assess genetic variation and its spatial structuring. Methods and Results: We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipelin...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
2016
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| Online Access: | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/31390 |
| _version_ | 1848753367322460160 |
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| author | Nevill, Paul Wardell-Johnson, G. |
| author_facet | Nevill, Paul Wardell-Johnson, G. |
| author_sort | Nevill, Paul |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Premise of the study: Microsatellite markers were developed for the rare sedge Lepidosperma bungalbin (Cyperaceae) to assess genetic variation and its spatial structuring. Methods and Results: We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. We then screened these loci for variation in individuals from three populations. The number of alleles observed for these 12 loci across the three populations ranged from nine to 19 and expected heterozygosity ranged from 0.41 to 0.89. Conclusions: These markers will enable the quantification of the potential impact of mining on genetic variation within L. bungalbin and establish a baseline for future management of genetic variation of the rare sedge. |
| first_indexed | 2025-11-14T08:23:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-31390 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:23:23Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-313902022-01-06T07:09:42Z Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) Nevill, Paul Wardell-Johnson, G. Premise of the study: Microsatellite markers were developed for the rare sedge Lepidosperma bungalbin (Cyperaceae) to assess genetic variation and its spatial structuring. Methods and Results: We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. We then screened these loci for variation in individuals from three populations. The number of alleles observed for these 12 loci across the three populations ranged from nine to 19 and expected heterozygosity ranged from 0.41 to 0.89. Conclusions: These markers will enable the quantification of the potential impact of mining on genetic variation within L. bungalbin and establish a baseline for future management of genetic variation of the rare sedge. 2016 Journal Article http://hdl.handle.net/20.500.11937/31390 10.3732/apps.1600083 http://purl.org/au-research/grants/arc/IC150100041 fulltext |
| spellingShingle | Nevill, Paul Wardell-Johnson, G. Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) |
| title | Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) |
| title_full | Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) |
| title_fullStr | Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) |
| title_full_unstemmed | Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) |
| title_short | Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae) |
| title_sort | microsatellite primers for the rare sedge lepidosperma bungalbin (cyperaceae) |
| url | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/31390 |