Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes

The use of sweet lupins as a new food is resulting in an increasing number of cases of allergy reactions, particularly in atopic patients with other pre-existing legume allergies. We performed an extensive in silico analysis of seed ß-conglutins, a new family of major allergen proteins in lupin, and...

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Main Authors: Jimenez-Lopez, J., Lima-Cabello, E., Melser, S., Foley, R., Singh, Karambir, Alché Juan, D.
Format: Conference Paper
Published: 2015
Online Access:http://hdl.handle.net/20.500.11937/31368
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author Jimenez-Lopez, J.
Lima-Cabello, E.
Melser, S.
Foley, R.
Singh, Karambir
Alché Juan, D.
author_facet Jimenez-Lopez, J.
Lima-Cabello, E.
Melser, S.
Foley, R.
Singh, Karambir
Alché Juan, D.
author_sort Jimenez-Lopez, J.
building Curtin Institutional Repository
collection Online Access
description The use of sweet lupins as a new food is resulting in an increasing number of cases of allergy reactions, particularly in atopic patients with other pre-existing legume allergies. We performed an extensive in silico analysis of seed ß-conglutins, a new family of major allergen proteins in lupin, and a comparison to other relevant food allergens such as Ara h 1. We analyzed surface residues involved in conformational epitopes, lineal B- and T-cell epitopes variability, and changes in 2-D structural elements and 3D motives, with the aim to investigate IgE-mediated cross-reactivity among lupin, peanut, and other different legumes. Our results revealed that considerable structural differences exist, particularly affecting 2-D elements (loops and coils), and numerous micro-heterogeneities are present in fundamental residues directly involved in epitopes variability. Variability of residues involved in IgE-binding epitopes might be a major contributor to the observed differences in cross-reactivity among legumes.
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spelling curtin-20.500.11937-313682017-09-19T07:42:10Z Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes Jimenez-Lopez, J. Lima-Cabello, E. Melser, S. Foley, R. Singh, Karambir Alché Juan, D. The use of sweet lupins as a new food is resulting in an increasing number of cases of allergy reactions, particularly in atopic patients with other pre-existing legume allergies. We performed an extensive in silico analysis of seed ß-conglutins, a new family of major allergen proteins in lupin, and a comparison to other relevant food allergens such as Ara h 1. We analyzed surface residues involved in conformational epitopes, lineal B- and T-cell epitopes variability, and changes in 2-D structural elements and 3D motives, with the aim to investigate IgE-mediated cross-reactivity among lupin, peanut, and other different legumes. Our results revealed that considerable structural differences exist, particularly affecting 2-D elements (loops and coils), and numerous micro-heterogeneities are present in fundamental residues directly involved in epitopes variability. Variability of residues involved in IgE-binding epitopes might be a major contributor to the observed differences in cross-reactivity among legumes. 2015 Conference Paper http://hdl.handle.net/20.500.11937/31368 10.1007/978-3-319-16483-0_10 restricted
spellingShingle Jimenez-Lopez, J.
Lima-Cabello, E.
Melser, S.
Foley, R.
Singh, Karambir
Alché Juan, D.
Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes
title Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes
title_full Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes
title_fullStr Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes
title_full_unstemmed Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes
title_short Lupin allergy: Uncovering structural features and epitopes of ß-conglutin proteins in Lupinus Angustifolius L. with a focus on cross-allergenic reactivity to peanut and other legumes
title_sort lupin allergy: uncovering structural features and epitopes of ß-conglutin proteins in lupinus angustifolius l. with a focus on cross-allergenic reactivity to peanut and other legumes
url http://hdl.handle.net/20.500.11937/31368