Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept

This study aimed to establish, as a proof of concept, whether bacterial cellulose (BC)–derived plant cell wall models could be used to investigate foodborne bacterial pathogen attachment. Attachment of two strains each of Salmonella enterica and Listeria monocytogenes to four BC–derived plant cell w...

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Main Authors: Tan, M., Wang, Y., Dykes, Gary
Format: Journal Article
Published: 2013
Online Access:http://hdl.handle.net/20.500.11937/31156
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author Tan, M.
Wang, Y.
Dykes, Gary
author_facet Tan, M.
Wang, Y.
Dykes, Gary
author_sort Tan, M.
building Curtin Institutional Repository
collection Online Access
description This study aimed to establish, as a proof of concept, whether bacterial cellulose (BC)–derived plant cell wall models could be used to investigate foodborne bacterial pathogen attachment. Attachment of two strains each of Salmonella enterica and Listeria monocytogenes to four BC–derived plant cell wall models (namely, BC, BC-pectin [BCP], BC-xyloglucan [BCX], and BC-pectin-xyloglucan [BCPX]) was investigated. Chemical analysis indicated that the BCPX composite (31% cellulose, 45.6% pectin, 23.4% xyloglucan) had a composition typical of plant cell walls. The Salmonella strains attached in significantly (p<0.05) higher numbers (~6 log colony-forming units [CFU]/cm2) to the composites than the Listeria strains (~5 log CFU/cm2). Strain-specific differences were also apparent with one Salmonella strain, for example, attaching in significantly (p<0.05) higher numbers to the BCX composite than to the other composites. This study highlights the potential usefulness of these composites to understand attachment of foodborne bacteria to fresh produce.
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spelling curtin-20.500.11937-311562017-09-13T15:13:59Z Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept Tan, M. Wang, Y. Dykes, Gary This study aimed to establish, as a proof of concept, whether bacterial cellulose (BC)–derived plant cell wall models could be used to investigate foodborne bacterial pathogen attachment. Attachment of two strains each of Salmonella enterica and Listeria monocytogenes to four BC–derived plant cell wall models (namely, BC, BC-pectin [BCP], BC-xyloglucan [BCX], and BC-pectin-xyloglucan [BCPX]) was investigated. Chemical analysis indicated that the BCPX composite (31% cellulose, 45.6% pectin, 23.4% xyloglucan) had a composition typical of plant cell walls. The Salmonella strains attached in significantly (p<0.05) higher numbers (~6 log colony-forming units [CFU]/cm2) to the composites than the Listeria strains (~5 log CFU/cm2). Strain-specific differences were also apparent with one Salmonella strain, for example, attaching in significantly (p<0.05) higher numbers to the BCX composite than to the other composites. This study highlights the potential usefulness of these composites to understand attachment of foodborne bacteria to fresh produce. 2013 Journal Article http://hdl.handle.net/20.500.11937/31156 10.1089/fpd.2013.1536 restricted
spellingShingle Tan, M.
Wang, Y.
Dykes, Gary
Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept
title Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept
title_full Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept
title_fullStr Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept
title_full_unstemmed Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept
title_short Attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: A proof of concept
title_sort attachment of bacterial pathogens to a bacterial cellulose-derived plant cell wall model: a proof of concept
url http://hdl.handle.net/20.500.11937/31156