Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions

In this work, the electrochemical behavior of nonredox-active poly-L-lysine dendrigraft molecules of four different generations was investigated at the interface between two immiscible electrolyte solutions (ITIES). The influence of the dendrigraft generation on the electrochemical response, sensiti...

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Main Authors: Herzog, G., Flynn, S., Johnson, C., Arrigan, Damien
Format: Journal Article
Published: American Chemical Society 2012
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/31074
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author Herzog, G.
Flynn, S.
Johnson, C.
Arrigan, Damien
author_facet Herzog, G.
Flynn, S.
Johnson, C.
Arrigan, Damien
author_sort Herzog, G.
building Curtin Institutional Repository
collection Online Access
description In this work, the electrochemical behavior of nonredox-active poly-L-lysine dendrigraft molecules of four different generations was investigated at the interface between two immiscible electrolyte solutions (ITIES). The influence of the dendrigraft generation on the electrochemical response, sensitivity of the calibration curves, and limit of detection was studied. Cyclic voltammetry at the ITIES revealed that the sensitivity increased (1840 to 25 800 nA µM-1) and the limit of detection decreased (11.10 to 0.65 µM) as the dendrigraft generation increased from generation G2 through to generation G5, respectively. The results are compared to those for protein voltammetry at the ITIES. Our studies suggest that the sensitivity expected for a synthetic ionized macromolecule can be predicted on the basis of its net charge and its diffusion coefficient. However, electrochemistry at the ITIES demonstrates a greater sensitivity toward proteins, which is attributed to their tertiary structure.
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institution Curtin University Malaysia
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publishDate 2012
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spelling curtin-20.500.11937-310742017-09-13T15:51:05Z Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions Herzog, G. Flynn, S. Johnson, C. Arrigan, Damien electroanalytical behavior In this work, the electrochemical behavior of nonredox-active poly-L-lysine dendrigraft molecules of four different generations was investigated at the interface between two immiscible electrolyte solutions (ITIES). The influence of the dendrigraft generation on the electrochemical response, sensitivity of the calibration curves, and limit of detection was studied. Cyclic voltammetry at the ITIES revealed that the sensitivity increased (1840 to 25 800 nA µM-1) and the limit of detection decreased (11.10 to 0.65 µM) as the dendrigraft generation increased from generation G2 through to generation G5, respectively. The results are compared to those for protein voltammetry at the ITIES. Our studies suggest that the sensitivity expected for a synthetic ionized macromolecule can be predicted on the basis of its net charge and its diffusion coefficient. However, electrochemistry at the ITIES demonstrates a greater sensitivity toward proteins, which is attributed to their tertiary structure. 2012 Journal Article http://hdl.handle.net/20.500.11937/31074 10.1021/ac300856w American Chemical Society restricted
spellingShingle electroanalytical behavior
Herzog, G.
Flynn, S.
Johnson, C.
Arrigan, Damien
Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions
title Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions
title_full Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions
title_fullStr Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions
title_full_unstemmed Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions
title_short Electroanalytical Behavior of Poly-L-Lysine Dendrigrafts at the Interface between Two Immiscible Electrolyte Solutions
title_sort electroanalytical behavior of poly-l-lysine dendrigrafts at the interface between two immiscible electrolyte solutions
topic electroanalytical behavior
url http://hdl.handle.net/20.500.11937/31074