Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique

© 2016 John Wiley & Sons Ltd. Objectives: To describe the prevalence of human rhinovirus (RV) species in children hospitalised with pneumonia in Manhiça, Mozambique, and the associations between RV species and demographic, clinical and laboratory features. Methods: Nasopharyngeal aspirates were...

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Main Authors: Annamalay, A., Lanaspa, M., Khoo, S., Madrid, L., Acácio, S., Zhang, Guicheng, Laing, I., Gern, J., Goldblatt, J., Bizzintino, J., Lehmann, D., Le Souëf, P., Bassat, Q.
Format: Journal Article
Published: Blackwell Publishing Ltd 2016
Online Access:http://hdl.handle.net/20.500.11937/3095
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author Annamalay, A.
Lanaspa, M.
Khoo, S.
Madrid, L.
Acácio, S.
Zhang, Guicheng
Laing, I.
Gern, J.
Goldblatt, J.
Bizzintino, J.
Lehmann, D.
Le Souëf, P.
Bassat, Q.
author_facet Annamalay, A.
Lanaspa, M.
Khoo, S.
Madrid, L.
Acácio, S.
Zhang, Guicheng
Laing, I.
Gern, J.
Goldblatt, J.
Bizzintino, J.
Lehmann, D.
Le Souëf, P.
Bassat, Q.
author_sort Annamalay, A.
building Curtin Institutional Repository
collection Online Access
description © 2016 John Wiley & Sons Ltd. Objectives: To describe the prevalence of human rhinovirus (RV) species in children hospitalised with pneumonia in Manhiça, Mozambique, and the associations between RV species and demographic, clinical and laboratory features. Methods: Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277) presenting to Manhiça District Hospital with clinical pneumonia. Blood samples were collected for HIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. A panel of common respiratory viruses was investigated using two independent multiplex RT-PCR assays with primers specific for each virus and viral type. RV species and genotypes were identified by seminested PCR assays, sequencing and phylogenetic tree analyses. Results: At least one respiratory virus was identified in 206 (74.4%) children hospitalised with clinical pneumonia. RV was the most common virus identified in both HIV-infected (17 of 38, 44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most common RV species identified (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275, 2.9%). Clinical presentation of the different RV species was similar and overlapping, with no particular species being associated with specific clinical features. Conclusions: RV-A and RV-C were the most common respiratory viruses identified in children hospitalised with clinical pneumonia in Manhiça. Clinical presentation of RV-A and RV-C was similar and overlapping.
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spelling curtin-20.500.11937-30952017-09-13T14:45:10Z Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique Annamalay, A. Lanaspa, M. Khoo, S. Madrid, L. Acácio, S. Zhang, Guicheng Laing, I. Gern, J. Goldblatt, J. Bizzintino, J. Lehmann, D. Le Souëf, P. Bassat, Q. © 2016 John Wiley & Sons Ltd. Objectives: To describe the prevalence of human rhinovirus (RV) species in children hospitalised with pneumonia in Manhiça, Mozambique, and the associations between RV species and demographic, clinical and laboratory features. Methods: Nasopharyngeal aspirates were collected from children 0 to 10 years of age (n = 277) presenting to Manhiça District Hospital with clinical pneumonia. Blood samples were collected for HIV and malaria testing, blood culture and full blood counts, and a chest X-ray was performed. A panel of common respiratory viruses was investigated using two independent multiplex RT-PCR assays with primers specific for each virus and viral type. RV species and genotypes were identified by seminested PCR assays, sequencing and phylogenetic tree analyses. Results: At least one respiratory virus was identified in 206 (74.4%) children hospitalised with clinical pneumonia. RV was the most common virus identified in both HIV-infected (17 of 38, 44.7%) and HIV-uninfected (74 of 237, 31.2%; P = 0.100) children. RV-A was the most common RV species identified (47 of 275, 17.0%), followed by RV-C (35/275, 12.6%) and RV-B (8/275, 2.9%). Clinical presentation of the different RV species was similar and overlapping, with no particular species being associated with specific clinical features. Conclusions: RV-A and RV-C were the most common respiratory viruses identified in children hospitalised with clinical pneumonia in Manhiça. Clinical presentation of RV-A and RV-C was similar and overlapping. 2016 Journal Article http://hdl.handle.net/20.500.11937/3095 10.1111/tmi.12743 Blackwell Publishing Ltd unknown
spellingShingle Annamalay, A.
Lanaspa, M.
Khoo, S.
Madrid, L.
Acácio, S.
Zhang, Guicheng
Laing, I.
Gern, J.
Goldblatt, J.
Bizzintino, J.
Lehmann, D.
Le Souëf, P.
Bassat, Q.
Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique
title Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique
title_full Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique
title_fullStr Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique
title_full_unstemmed Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique
title_short Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique
title_sort rhinovirus species and clinical features in children hospitalised with pneumonia from mozambique
url http://hdl.handle.net/20.500.11937/3095