Liver progenitor cell interactions with the extracellular matrix
Liver progenitor cells (LPCs) are a promising source of cells to treat liver disease by cell therapy, due to their capability for self-replication and bipotentiality. In order to establish useful culture systems of LPCs and apply them to future clinical therapies, it is necessary to understand their...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
John Wiley & Sons, Inc.
2012
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/30858 |
| _version_ | 1848753209932251136 |
|---|---|
| author | Zhu, C. Coombe, Deirdre Zheng, M. Yeoh, G. Li, L. |
| author_facet | Zhu, C. Coombe, Deirdre Zheng, M. Yeoh, G. Li, L. |
| author_sort | Zhu, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Liver progenitor cells (LPCs) are a promising source of cells to treat liver disease by cell therapy, due to their capability for self-replication and bipotentiality. In order to establish useful culture systems of LPCs and apply them to future clinical therapies, it is necessary to understand their interactions with their microenvironment and especially with the extracellular matrix (ECM). There is considerable evidence from in vivo studies that matrix proteins affect the activation, expansion, migration and differentiation of LPCs, but the information on the role that specific ECMs play in regulating LPCs in vitro is more limited. Nevertheless, current studies suggest that laminin, collagen type III, collagen type IV and hyaluronic acid help to maintain the undifferentiated phenotype of LPCs and promote their proliferation when cultured in media supplemented with growth factors chosen for LPC expansion, whereas collagen type I and fibronectin are generally associated with a differentiated phenotype under the same conditions. Experimental evidence suggests that a6ß1 and a5ß1 integrins as well as CD44 on the surface of LPCs, and their related downstream signals, are important mediators of interactions between LPCs and the ECM. The interactions of LPCs with the ECM form the focus of this review and the contribution of ECM molecules to strategies for optimizing in vitro LPC cultures for therapeutic applications is discussed. |
| first_indexed | 2025-11-14T08:20:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-30858 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:20:53Z |
| publishDate | 2012 |
| publisher | John Wiley & Sons, Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-308582017-09-13T15:10:44Z Liver progenitor cell interactions with the extracellular matrix Zhu, C. Coombe, Deirdre Zheng, M. Yeoh, G. Li, L. extracellular matrix matrix receptors integrins matrix turnover liver progenitor cells interactions Liver progenitor cells (LPCs) are a promising source of cells to treat liver disease by cell therapy, due to their capability for self-replication and bipotentiality. In order to establish useful culture systems of LPCs and apply them to future clinical therapies, it is necessary to understand their interactions with their microenvironment and especially with the extracellular matrix (ECM). There is considerable evidence from in vivo studies that matrix proteins affect the activation, expansion, migration and differentiation of LPCs, but the information on the role that specific ECMs play in regulating LPCs in vitro is more limited. Nevertheless, current studies suggest that laminin, collagen type III, collagen type IV and hyaluronic acid help to maintain the undifferentiated phenotype of LPCs and promote their proliferation when cultured in media supplemented with growth factors chosen for LPC expansion, whereas collagen type I and fibronectin are generally associated with a differentiated phenotype under the same conditions. Experimental evidence suggests that a6ß1 and a5ß1 integrins as well as CD44 on the surface of LPCs, and their related downstream signals, are important mediators of interactions between LPCs and the ECM. The interactions of LPCs with the ECM form the focus of this review and the contribution of ECM molecules to strategies for optimizing in vitro LPC cultures for therapeutic applications is discussed. 2012 Journal Article http://hdl.handle.net/20.500.11937/30858 10.1002/term.1470 John Wiley & Sons, Inc. restricted |
| spellingShingle | extracellular matrix matrix receptors integrins matrix turnover liver progenitor cells interactions Zhu, C. Coombe, Deirdre Zheng, M. Yeoh, G. Li, L. Liver progenitor cell interactions with the extracellular matrix |
| title | Liver progenitor cell interactions with the extracellular matrix |
| title_full | Liver progenitor cell interactions with the extracellular matrix |
| title_fullStr | Liver progenitor cell interactions with the extracellular matrix |
| title_full_unstemmed | Liver progenitor cell interactions with the extracellular matrix |
| title_short | Liver progenitor cell interactions with the extracellular matrix |
| title_sort | liver progenitor cell interactions with the extracellular matrix |
| topic | extracellular matrix matrix receptors integrins matrix turnover liver progenitor cells interactions |
| url | http://hdl.handle.net/20.500.11937/30858 |