An integrated microspectrometer for localised multiplexing measurements
We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Royal Society of Chemistry
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/30681 |
| _version_ | 1848753157875695616 |
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| author | Hu, Z. Glidle, A. Ironside, Charlie Cooper, J. Yin, H. |
| author_facet | Hu, Z. Glidle, A. Ironside, Charlie Cooper, J. Yin, H. |
| author_sort | Hu, Z. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location. This journal is |
| first_indexed | 2025-11-14T08:20:03Z |
| format | Journal Article |
| id | curtin-20.500.11937-30681 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:20:03Z |
| publishDate | 2015 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-306812017-09-13T15:06:34Z An integrated microspectrometer for localised multiplexing measurements Hu, Z. Glidle, A. Ironside, Charlie Cooper, J. Yin, H. We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location. This journal is 2015 Journal Article http://hdl.handle.net/20.500.11937/30681 10.1039/c4lc00952e Royal Society of Chemistry fulltext |
| spellingShingle | Hu, Z. Glidle, A. Ironside, Charlie Cooper, J. Yin, H. An integrated microspectrometer for localised multiplexing measurements |
| title | An integrated microspectrometer for localised multiplexing measurements |
| title_full | An integrated microspectrometer for localised multiplexing measurements |
| title_fullStr | An integrated microspectrometer for localised multiplexing measurements |
| title_full_unstemmed | An integrated microspectrometer for localised multiplexing measurements |
| title_short | An integrated microspectrometer for localised multiplexing measurements |
| title_sort | integrated microspectrometer for localised multiplexing measurements |
| url | http://hdl.handle.net/20.500.11937/30681 |