Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys
© 2015. The vascular system particularly endothelium is sensitive to ischemia-reperfusion (I/R) injury, which is a big challenge in surgical practices and many vascular disorders. In the present study, we reported the global gene expression changes in a 2-h ischemia and 4-h reperfusion injury induce...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
2015
|
| Online Access: | http://hdl.handle.net/20.500.11937/30375 |
| _version_ | 1848753071505539072 |
|---|---|
| author | Luo, R. Li, L. Du, X. Shi, M. Zhou, C. Wang, C. Liao, G. Lu, Y. Zhong, Z. Cheng, J. Chen, Younan |
| author_facet | Luo, R. Li, L. Du, X. Shi, M. Zhou, C. Wang, C. Liao, G. Lu, Y. Zhong, Z. Cheng, J. Chen, Younan |
| author_sort | Luo, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2015. The vascular system particularly endothelium is sensitive to ischemia-reperfusion (I/R) injury, which is a big challenge in surgical practices and many vascular disorders. In the present study, we reported the global gene expression changes in a 2-h ischemia and 4-h reperfusion injury induced in the hind limb vessels of rhesus monkeys (Macaca mulatta) using microarray technique. Results: The histological results showed abnormal morphology of endothelial cells after 2-h ischemia and the hematological detection found slightly extension of coagulation time after I/R treatment. Furthermore, we found distinct alterations in gene expression patterns during I/R process. These identified genes are mostly involved in inflammation, immune response, apoptosis, and cell stress signaling pathways. The significantly up-regulated genes included IL-6, regulator of G-protein signaling 8, selectin E, and metallothionein 2A, et al. Whist, the robustly down-regulated genes included NECAP endocytosis associated 2, transglutaminase 2, and fibronectin 1, et al. Conclusion: Our results indicate that inflammation, primarily characterized by gene expression changes of cytokines and chemokines is the most important event in the early stage of I/R injury in blood vessels. |
| first_indexed | 2025-11-14T08:18:41Z |
| format | Journal Article |
| id | curtin-20.500.11937-30375 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:18:41Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-303752017-09-13T15:31:15Z Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys Luo, R. Li, L. Du, X. Shi, M. Zhou, C. Wang, C. Liao, G. Lu, Y. Zhong, Z. Cheng, J. Chen, Younan © 2015. The vascular system particularly endothelium is sensitive to ischemia-reperfusion (I/R) injury, which is a big challenge in surgical practices and many vascular disorders. In the present study, we reported the global gene expression changes in a 2-h ischemia and 4-h reperfusion injury induced in the hind limb vessels of rhesus monkeys (Macaca mulatta) using microarray technique. Results: The histological results showed abnormal morphology of endothelial cells after 2-h ischemia and the hematological detection found slightly extension of coagulation time after I/R treatment. Furthermore, we found distinct alterations in gene expression patterns during I/R process. These identified genes are mostly involved in inflammation, immune response, apoptosis, and cell stress signaling pathways. The significantly up-regulated genes included IL-6, regulator of G-protein signaling 8, selectin E, and metallothionein 2A, et al. Whist, the robustly down-regulated genes included NECAP endocytosis associated 2, transglutaminase 2, and fibronectin 1, et al. Conclusion: Our results indicate that inflammation, primarily characterized by gene expression changes of cytokines and chemokines is the most important event in the early stage of I/R injury in blood vessels. 2015 Journal Article http://hdl.handle.net/20.500.11937/30375 10.1016/j.gene.2015.10.073 restricted |
| spellingShingle | Luo, R. Li, L. Du, X. Shi, M. Zhou, C. Wang, C. Liao, G. Lu, Y. Zhong, Z. Cheng, J. Chen, Younan Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| title | Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| title_full | Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| title_fullStr | Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| title_full_unstemmed | Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| title_short | Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| title_sort | gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys |
| url | http://hdl.handle.net/20.500.11937/30375 |