Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface
Electrochemical characterization of hen egg white lysozyme (HEWL) at a glycerol-modified interface between two immiscible electrolyte solutions (ITIES) was conducted using a microporous silicon membrane-supported gelled-1,6-dichlorohexane|water-glycerol interface. The electrochemical response of HEW...
| Main Authors: | , |
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| Format: | Journal Article |
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Pergamon
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/2940 |
| _version_ | 1848744091116896256 |
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| author | Austen, Benjamin Arrigan, Damien |
| author_facet | Austen, Benjamin Arrigan, Damien |
| author_sort | Austen, Benjamin |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Electrochemical characterization of hen egg white lysozyme (HEWL) at a glycerol-modified interface between two immiscible electrolyte solutions (ITIES) was conducted using a microporous silicon membrane-supported gelled-1,6-dichlorohexane|water-glycerol interface. The electrochemical response of HEWL under these conditions is of interest for the system's potential application to the formation of isorefractive emulsified-ITIES, which offer practical opportunities for spectrophotometric analysis of interfacial processes. Importantly, the voltammetric signature for HEWL seen under glycerol-rich conditions was similar but with some differences from that for glycerol-free conditions. Specifically, the potential at which facilitated transfer of the organic phase electrolyte anion tetrakis-(4-chlorophenyl) borate (TPBCl-) occurred was shifted to lower potential with increasing glycerol concentration. However, features in the voltammetry associated with adsorption/desorption processes were observed to remain constant. The simple ion transfer response of tetraethylammonium cations (TEA+) at the same glycerol-modified ITIES provides insight into the nature of changes that determine the atypical HEWL signature. Lower ion transfer current with respect to increasing glycerol concentration and a shift in transfer potential were the key findings here. The results indicate that the electrochemistry which determines the HEWL signature is similar in environments that are rich in glycerol or purely aqueous. |
| first_indexed | 2025-11-14T05:55:57Z |
| format | Journal Article |
| id | curtin-20.500.11937-2940 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T05:55:57Z |
| publishDate | 2016 |
| publisher | Pergamon |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-29402018-10-18T01:45:59Z Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface Austen, Benjamin Arrigan, Damien Electrochemical characterization of hen egg white lysozyme (HEWL) at a glycerol-modified interface between two immiscible electrolyte solutions (ITIES) was conducted using a microporous silicon membrane-supported gelled-1,6-dichlorohexane|water-glycerol interface. The electrochemical response of HEWL under these conditions is of interest for the system's potential application to the formation of isorefractive emulsified-ITIES, which offer practical opportunities for spectrophotometric analysis of interfacial processes. Importantly, the voltammetric signature for HEWL seen under glycerol-rich conditions was similar but with some differences from that for glycerol-free conditions. Specifically, the potential at which facilitated transfer of the organic phase electrolyte anion tetrakis-(4-chlorophenyl) borate (TPBCl-) occurred was shifted to lower potential with increasing glycerol concentration. However, features in the voltammetry associated with adsorption/desorption processes were observed to remain constant. The simple ion transfer response of tetraethylammonium cations (TEA+) at the same glycerol-modified ITIES provides insight into the nature of changes that determine the atypical HEWL signature. Lower ion transfer current with respect to increasing glycerol concentration and a shift in transfer potential were the key findings here. The results indicate that the electrochemistry which determines the HEWL signature is similar in environments that are rich in glycerol or purely aqueous. 2016 Journal Article http://hdl.handle.net/20.500.11937/2940 10.1016/j.electacta.2016.10.098 Pergamon fulltext |
| spellingShingle | Austen, Benjamin Arrigan, Damien Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| title | Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| title_full | Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| title_fullStr | Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| title_full_unstemmed | Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| title_short | Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| title_sort | electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface |
| url | http://hdl.handle.net/20.500.11937/2940 |