The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial

Iron is implicated in the pathogenesis of liver injury and insulin resistance (IR) and thus phlebotomy has been proposed as a treatment for nonalcoholic fatty liver disease (NAFLD). We performed a prospective 6-month randomized, controlled trial examining the impact of phlebotomy on the background o...

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Main Authors: Adams, L., Crawford, D., Stuart, K., House, M., St Pierre, T., Webb, M., Ching, H., Kava, J., Bynevelt, M., Macquillan, G., Garas, G., Ayonrinde, Oyekoya, Mori, T., Croft, K., Niu, X., Jeffrey, G., Olynyk, John
Format: Journal Article
Published: John Wiley and Sons Inc. 2015
Online Access:http://hdl.handle.net/20.500.11937/29260
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author Adams, L.
Crawford, D.
Stuart, K.
House, M.
St Pierre, T.
Webb, M.
Ching, H.
Kava, J.
Bynevelt, M.
Macquillan, G.
Garas, G.
Ayonrinde, Oyekoya
Mori, T.
Croft, K.
Niu, X.
Jeffrey, G.
Olynyk, John
author_facet Adams, L.
Crawford, D.
Stuart, K.
House, M.
St Pierre, T.
Webb, M.
Ching, H.
Kava, J.
Bynevelt, M.
Macquillan, G.
Garas, G.
Ayonrinde, Oyekoya
Mori, T.
Croft, K.
Niu, X.
Jeffrey, G.
Olynyk, John
author_sort Adams, L.
building Curtin Institutional Repository
collection Online Access
description Iron is implicated in the pathogenesis of liver injury and insulin resistance (IR) and thus phlebotomy has been proposed as a treatment for nonalcoholic fatty liver disease (NAFLD). We performed a prospective 6-month randomized, controlled trial examining the impact of phlebotomy on the background of lifestyle advice in patients with NAFLD. Primary endpoints were hepatic steatosis (HS; quantified by magnetic resonance imaging) and liver injury (determined by alanine aminotransaminase [ALT] and cytokeratin-18 [CK-18]). Secondary endpoints included insulin resistance measured by the insulin sensitivity index (ISI) and homeostasis model of assessment (HOMA), and systemic lipid peroxidation determined by plasma F2-isoprostane levels. A total of 74 subjects were randomized (33 phlebotomy and 41 control). The phlebotomy group underwent a median (range) of 7 (1-19) venesection sessions and had a significantly greater reduction in ferritin levels over 6 months, compared to controls (-148±114 vs. -38±89 ng/mL; P<0.001). At 6 months, there was no difference between phlebotomy and control groups in HS (17.7% vs. 15.5%; P=0.4), serum ALT (36 vs. 46 IU/L; P=0.4), or CK-18 levels (175 vs. 196 U/L; P=0.9). Similarly, there was no difference in end-of-study ISI (2.5 vs. 2.7; P=0.9), HOMA (3.2 vs. 3.2; P=0.6), or F2-isoprostane levels (1,332 vs. 1,190 pmmol/L; P=0.6) between phlebotomy and control groups. No differences in any endpoint were noted in patients with hyperferritinemia at baseline. Among patients undergoing phlebotomy, there was no correlation between number of phlebotomy sessions and change in HS, liver injury, or IR from baseline to end of study. Conclusion: Reduction in ferritin by phlebotomy does not improve liver enzymes, hepatic fat, or IR in subjects with NAFLD.
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spelling curtin-20.500.11937-292602017-09-13T15:22:41Z The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial Adams, L. Crawford, D. Stuart, K. House, M. St Pierre, T. Webb, M. Ching, H. Kava, J. Bynevelt, M. Macquillan, G. Garas, G. Ayonrinde, Oyekoya Mori, T. Croft, K. Niu, X. Jeffrey, G. Olynyk, John Iron is implicated in the pathogenesis of liver injury and insulin resistance (IR) and thus phlebotomy has been proposed as a treatment for nonalcoholic fatty liver disease (NAFLD). We performed a prospective 6-month randomized, controlled trial examining the impact of phlebotomy on the background of lifestyle advice in patients with NAFLD. Primary endpoints were hepatic steatosis (HS; quantified by magnetic resonance imaging) and liver injury (determined by alanine aminotransaminase [ALT] and cytokeratin-18 [CK-18]). Secondary endpoints included insulin resistance measured by the insulin sensitivity index (ISI) and homeostasis model of assessment (HOMA), and systemic lipid peroxidation determined by plasma F2-isoprostane levels. A total of 74 subjects were randomized (33 phlebotomy and 41 control). The phlebotomy group underwent a median (range) of 7 (1-19) venesection sessions and had a significantly greater reduction in ferritin levels over 6 months, compared to controls (-148±114 vs. -38±89 ng/mL; P<0.001). At 6 months, there was no difference between phlebotomy and control groups in HS (17.7% vs. 15.5%; P=0.4), serum ALT (36 vs. 46 IU/L; P=0.4), or CK-18 levels (175 vs. 196 U/L; P=0.9). Similarly, there was no difference in end-of-study ISI (2.5 vs. 2.7; P=0.9), HOMA (3.2 vs. 3.2; P=0.6), or F2-isoprostane levels (1,332 vs. 1,190 pmmol/L; P=0.6) between phlebotomy and control groups. No differences in any endpoint were noted in patients with hyperferritinemia at baseline. Among patients undergoing phlebotomy, there was no correlation between number of phlebotomy sessions and change in HS, liver injury, or IR from baseline to end of study. Conclusion: Reduction in ferritin by phlebotomy does not improve liver enzymes, hepatic fat, or IR in subjects with NAFLD. 2015 Journal Article http://hdl.handle.net/20.500.11937/29260 10.1002/hep.27662 John Wiley and Sons Inc. unknown
spellingShingle Adams, L.
Crawford, D.
Stuart, K.
House, M.
St Pierre, T.
Webb, M.
Ching, H.
Kava, J.
Bynevelt, M.
Macquillan, G.
Garas, G.
Ayonrinde, Oyekoya
Mori, T.
Croft, K.
Niu, X.
Jeffrey, G.
Olynyk, John
The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial
title The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial
title_full The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial
title_fullStr The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial
title_full_unstemmed The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial
title_short The impact of phlebotomy in nonalcoholic fatty liver disease: A prospective, randomized, controlled trial
title_sort impact of phlebotomy in nonalcoholic fatty liver disease: a prospective, randomized, controlled trial
url http://hdl.handle.net/20.500.11937/29260