Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays

Geometrically regular silicon membrane-based micropore arrays were employed for defined arrays of micrometer-sized interfaces between two immiscible electrolyte solutions (μITIES). These were incorporated into a poly(tetrafluoroethylene) (PTFE) hydrodynamic cell. Electrochemistry at the μITIES array...

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Main Authors: Scanlon, M., Berduque, A., Strutwolf, J., Arrigan, Damien
Format: Journal Article
Published: Pergamon-Elsevier Science Ltd 2010
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/28837
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author Scanlon, M.
Berduque, A.
Strutwolf, J.
Arrigan, Damien
author_facet Scanlon, M.
Berduque, A.
Strutwolf, J.
Arrigan, Damien
author_sort Scanlon, M.
building Curtin Institutional Repository
collection Online Access
description Geometrically regular silicon membrane-based micropore arrays were employed for defined arrays of micrometer-sized interfaces between two immiscible electrolyte solutions (μITIES). These were incorporated into a poly(tetrafluoroethylene) (PTFE) hydrodynamic cell. Electrochemistry at the μITIES array was undertaken following gellification of the organic phase using polyvinyl chloride (PVC) and flowing an aqueous phase over the array surface. Cyclic voltammetric characterization of asymmetric diffusion profiles on either side of the μITIES was accomplished under flowing conditions using positively and negatively charged (TEA+ and 4-OBSA−, respectively) model analyte species. Incorporation of an ionophore (dibenzo-18-crown-6 ether) into the organogel allowed the ion-transfer detection of two oligopeptides (phenylalanine dipeptide and lysine dipeptide) within the available potential window under stationary and flowing conditions.Flow rate studies with TEA+ indicated that the amperometric peak currents do not obey the Levich equation, due to diffusion dominating the mass transport, as opposed to convection. The influence of the applied potential () on the amperometric response of the oligopeptides was studied and hydrodynamic voltammograms (HDVs) for the individual oligopeptides were subsequently constructed. The data presented provide a basis for the use of silicon membrane-based μITIES arrays in flow analytical methods.
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spelling curtin-20.500.11937-288372019-02-19T04:28:03Z Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays Scanlon, M. Berduque, A. Strutwolf, J. Arrigan, Damien Liquid–liquid interface Amperometry Micropores Oligopeptides Hydrodynamic voltammogram Flow-injection analysis Geometrically regular silicon membrane-based micropore arrays were employed for defined arrays of micrometer-sized interfaces between two immiscible electrolyte solutions (μITIES). These were incorporated into a poly(tetrafluoroethylene) (PTFE) hydrodynamic cell. Electrochemistry at the μITIES array was undertaken following gellification of the organic phase using polyvinyl chloride (PVC) and flowing an aqueous phase over the array surface. Cyclic voltammetric characterization of asymmetric diffusion profiles on either side of the μITIES was accomplished under flowing conditions using positively and negatively charged (TEA+ and 4-OBSA−, respectively) model analyte species. Incorporation of an ionophore (dibenzo-18-crown-6 ether) into the organogel allowed the ion-transfer detection of two oligopeptides (phenylalanine dipeptide and lysine dipeptide) within the available potential window under stationary and flowing conditions.Flow rate studies with TEA+ indicated that the amperometric peak currents do not obey the Levich equation, due to diffusion dominating the mass transport, as opposed to convection. The influence of the applied potential () on the amperometric response of the oligopeptides was studied and hydrodynamic voltammograms (HDVs) for the individual oligopeptides were subsequently constructed. The data presented provide a basis for the use of silicon membrane-based μITIES arrays in flow analytical methods. 2010 Journal Article http://hdl.handle.net/20.500.11937/28837 10.1016/j.electacta.2008.12.014 Pergamon-Elsevier Science Ltd fulltext
spellingShingle Liquid–liquid interface
Amperometry
Micropores
Oligopeptides
Hydrodynamic voltammogram
Flow-injection analysis
Scanlon, M.
Berduque, A.
Strutwolf, J.
Arrigan, Damien
Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
title Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
title_full Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
title_fullStr Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
title_full_unstemmed Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
title_short Flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
title_sort flow-injection amperometry at microfabricated silicon-based μ-liquid–liquid interface arrays
topic Liquid–liquid interface
Amperometry
Micropores
Oligopeptides
Hydrodynamic voltammogram
Flow-injection analysis
url http://hdl.handle.net/20.500.11937/28837