Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation
In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endoto...
| Main Authors: | , |
|---|---|
| Format: | Journal Article |
| Published: |
Taylor & Francis Inc.
2014
|
| Online Access: | http://hdl.handle.net/20.500.11937/28276 |
| _version_ | 1848752492381208576 |
|---|---|
| author | Ongkudon, C. Danquah, Michael |
| author_facet | Ongkudon, C. Danquah, Michael |
| author_sort | Ongkudon, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endotoxin assay. This work concludes that Zn2+ (as ZnSO4) optimally binds to and increases the hydrodynamic size of endotoxins compared to Mg2+ and Ca2+. A low polydispersity index value measured after cation addition indicates the presence of homogenously suspended supramolecular endotoxin particles which calls for a centrifugal separation step for complete endotoxin removal. © 2014 Copyright Taylor and Francis Group, LLC. |
| first_indexed | 2025-11-14T08:09:29Z |
| format | Journal Article |
| id | curtin-20.500.11937-28276 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:09:29Z |
| publishDate | 2014 |
| publisher | Taylor & Francis Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-282762018-03-29T09:08:50Z Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation Ongkudon, C. Danquah, Michael In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endotoxin assay. This work concludes that Zn2+ (as ZnSO4) optimally binds to and increases the hydrodynamic size of endotoxins compared to Mg2+ and Ca2+. A low polydispersity index value measured after cation addition indicates the presence of homogenously suspended supramolecular endotoxin particles which calls for a centrifugal separation step for complete endotoxin removal. © 2014 Copyright Taylor and Francis Group, LLC. 2014 Journal Article http://hdl.handle.net/20.500.11937/28276 10.1080/01496395.2013.835825 Taylor & Francis Inc. restricted |
| spellingShingle | Ongkudon, C. Danquah, Michael Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation |
| title | Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation |
| title_full | Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation |
| title_fullStr | Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation |
| title_full_unstemmed | Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation |
| title_short | Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation |
| title_sort | analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation |
| url | http://hdl.handle.net/20.500.11937/28276 |