Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon
Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon guttatissimus), which are widely distributed in the Western Pacific and Indian...
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| Format: | Journal Article |
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Springer
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/2817 |
| _version_ | 1848744057521569792 |
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| author | Montanari, S. Gardener, M. Hobbs, Jean-Paul Prachett, M. Bay, L. Van Herwerden, L. |
| author_facet | Montanari, S. Gardener, M. Hobbs, Jean-Paul Prachett, M. Bay, L. Van Herwerden, L. |
| author_sort | Montanari, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon guttatissimus), which are widely distributed in the Western Pacific and Indian Ocean, respectively. All loci were genotyped in samples collected from Christmas Island: C. guttatissimus (n = 25), C. punctatofasciatus (n = 17) and hybrids (n = 16). Mean alleles per locus (N a) were: 9.05 for C. guttatissimus, 9.95 for C. punctatofasciatus and 9.45 for hybrids. Observed heterozygosity (H O) ranged from 0.00 to 1.00 for C. guttatissimus; from 0.08 to 0.88 for C. punctatofasciatus; and from 0.19 to 0.94 for hybrids. Most loci conformed to Hardy-Weinberg expectations, were in linkage equilibrium, and did not contain null alleles. These markers will be useful for testing population genetic hypotheses including patterns of hybridization in this pair of butterflyfishes. |
| first_indexed | 2025-11-14T05:55:25Z |
| format | Journal Article |
| id | curtin-20.500.11937-2817 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T05:55:25Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-28172017-09-13T14:31:38Z Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon Montanari, S. Gardener, M. Hobbs, Jean-Paul Prachett, M. Bay, L. Van Herwerden, L. Christmas Island Suture zone Butterflyfish Allopatry Indo-Pacific Hybridization Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon guttatissimus), which are widely distributed in the Western Pacific and Indian Ocean, respectively. All loci were genotyped in samples collected from Christmas Island: C. guttatissimus (n = 25), C. punctatofasciatus (n = 17) and hybrids (n = 16). Mean alleles per locus (N a) were: 9.05 for C. guttatissimus, 9.95 for C. punctatofasciatus and 9.45 for hybrids. Observed heterozygosity (H O) ranged from 0.00 to 1.00 for C. guttatissimus; from 0.08 to 0.88 for C. punctatofasciatus; and from 0.19 to 0.94 for hybrids. Most loci conformed to Hardy-Weinberg expectations, were in linkage equilibrium, and did not contain null alleles. These markers will be useful for testing population genetic hypotheses including patterns of hybridization in this pair of butterflyfishes. 2013 Journal Article http://hdl.handle.net/20.500.11937/2817 10.1007/s12686-013-9907-7 Springer restricted |
| spellingShingle | Christmas Island Suture zone Butterflyfish Allopatry Indo-Pacific Hybridization Montanari, S. Gardener, M. Hobbs, Jean-Paul Prachett, M. Bay, L. Van Herwerden, L. Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon |
| title | Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon |
| title_full | Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon |
| title_fullStr | Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon |
| title_full_unstemmed | Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon |
| title_short | Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon |
| title_sort | isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus chaetodon |
| topic | Christmas Island Suture zone Butterflyfish Allopatry Indo-Pacific Hybridization |
| url | http://hdl.handle.net/20.500.11937/2817 |