Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics
Antimicrobial peptides (AMPs) play a vital role in defense against resistant bacteria. In this study, eight different AMPs synthesized from Python reticulatus serum protein were tested for bactericidal activity against various Gram-positive and Gram-negative bacteria (Staphylococcus aureus, Burkhold...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/28144 |
| _version_ | 1848752456761081856 |
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| author | Samy, R. Thwin, M. Stiles, B. Satyanarayana-Jois, S. Chinnathambi, A. Zayed, M. Alharbi, S. Siveen, K. Sikka, S. Kumar, Alan Prem Sethi, G. Lim, L. |
| author_facet | Samy, R. Thwin, M. Stiles, B. Satyanarayana-Jois, S. Chinnathambi, A. Zayed, M. Alharbi, S. Siveen, K. Sikka, S. Kumar, Alan Prem Sethi, G. Lim, L. |
| author_sort | Samy, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Antimicrobial peptides (AMPs) play a vital role in defense against resistant bacteria. In this study, eight different AMPs synthesized from Python reticulatus serum protein were tested for bactericidal activity against various Gram-positive and Gram-negative bacteria (Staphylococcus aureus, Burkholderia pseudomallei (KHW and TES strains), and Proteus vulgaris) using a disc-diffusion method (20 µg/disc). Among the tested peptides, phospholipase A2 inhibitory peptide (PIP)-18[59-76], ß-Asp65-PIP[59-67], D-Ala66-PNT.II, and D60,65E-PIP[59-67] displayed the most potent bactericidal activity against all tested pathogens in a dose-dependent manner (100-6.8 µg/ml), with a remarkable activity noted against S. aureus at 6.8 µg/ml dose within 6 h of incubation. Determination of minimum inhibitory concentrations (MICs) by a micro-broth dilution method at 100-3.125 µg/ml revealed that PIP-18[59-76], ß-Asp65-PIP[59-67] and D-Ala66-PNT.II peptides exerted a potent inhibitory effect against S. aureus and B. pseudomallei (KHW) (MICs 3.125 µg/ml), while a much less inhibitory potency (MICs 12.5 µg/ml) was noted for ß-Asp65-PIP[59-67] and D-Ala66-PNT.II peptides against B. pseudomallei (TES). Higher doses of peptides had no effect on the other two strains (i.e., Klebsiella pneumoniae and Streptococcus pneumoniae).Overall, PIP-18[59-76] possessed higher antimicrobial activity than that of chloramphenicol (CHL), ceftazidime (CF) and streptomycin (ST) (30 µg/disc). When the two most active peptides, PIP-18[59-76] and ß-Asp65-PIP[59-67], were applied topically at a 150 mg/kg dose for testing wound healing activity in a mouse model of S. aureus infection, the former accelerates faster wound healing than the latter peptide at 14 days post-treatment. The western blot data suggest that the topical application of peptides (PIP-18[59-67] and ß-Asp65-PIP[59-67]) modulates NF-kB mediated wound repair in mice with relatively little haemolytic (100-1.56 µg/ml) and cytotoxic (1000-3.125 µg/ml) effects evident on human cells in vitro. |
| first_indexed | 2025-11-14T08:08:55Z |
| format | Journal Article |
| id | curtin-20.500.11937-28144 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:08:55Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-281442017-09-13T15:12:27Z Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics Samy, R. Thwin, M. Stiles, B. Satyanarayana-Jois, S. Chinnathambi, A. Zayed, M. Alharbi, S. Siveen, K. Sikka, S. Kumar, Alan Prem Sethi, G. Lim, L. Antimicrobial peptides (AMPs) play a vital role in defense against resistant bacteria. In this study, eight different AMPs synthesized from Python reticulatus serum protein were tested for bactericidal activity against various Gram-positive and Gram-negative bacteria (Staphylococcus aureus, Burkholderia pseudomallei (KHW and TES strains), and Proteus vulgaris) using a disc-diffusion method (20 µg/disc). Among the tested peptides, phospholipase A2 inhibitory peptide (PIP)-18[59-76], ß-Asp65-PIP[59-67], D-Ala66-PNT.II, and D60,65E-PIP[59-67] displayed the most potent bactericidal activity against all tested pathogens in a dose-dependent manner (100-6.8 µg/ml), with a remarkable activity noted against S. aureus at 6.8 µg/ml dose within 6 h of incubation. Determination of minimum inhibitory concentrations (MICs) by a micro-broth dilution method at 100-3.125 µg/ml revealed that PIP-18[59-76], ß-Asp65-PIP[59-67] and D-Ala66-PNT.II peptides exerted a potent inhibitory effect against S. aureus and B. pseudomallei (KHW) (MICs 3.125 µg/ml), while a much less inhibitory potency (MICs 12.5 µg/ml) was noted for ß-Asp65-PIP[59-67] and D-Ala66-PNT.II peptides against B. pseudomallei (TES). Higher doses of peptides had no effect on the other two strains (i.e., Klebsiella pneumoniae and Streptococcus pneumoniae).Overall, PIP-18[59-76] possessed higher antimicrobial activity than that of chloramphenicol (CHL), ceftazidime (CF) and streptomycin (ST) (30 µg/disc). When the two most active peptides, PIP-18[59-76] and ß-Asp65-PIP[59-67], were applied topically at a 150 mg/kg dose for testing wound healing activity in a mouse model of S. aureus infection, the former accelerates faster wound healing than the latter peptide at 14 days post-treatment. The western blot data suggest that the topical application of peptides (PIP-18[59-67] and ß-Asp65-PIP[59-67]) modulates NF-kB mediated wound repair in mice with relatively little haemolytic (100-1.56 µg/ml) and cytotoxic (1000-3.125 µg/ml) effects evident on human cells in vitro. 2015 Journal Article http://hdl.handle.net/20.500.11937/28144 10.1016/j.biochi.2015.01.003 Elsevier restricted |
| spellingShingle | Samy, R. Thwin, M. Stiles, B. Satyanarayana-Jois, S. Chinnathambi, A. Zayed, M. Alharbi, S. Siveen, K. Sikka, S. Kumar, Alan Prem Sethi, G. Lim, L. Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics |
| title | Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics |
| title_full | Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics |
| title_fullStr | Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics |
| title_full_unstemmed | Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics |
| title_short | Novel phospholipase A2 inhibitors from python serum are potent peptide antibiotics |
| title_sort | novel phospholipase a2 inhibitors from python serum are potent peptide antibiotics |
| url | http://hdl.handle.net/20.500.11937/28144 |