Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi
Endemic species at remote islands have a high risk of extinction because they often exhibit ecological, biological and genetic traits that make them particularly vulnerable to disturbances. McCulloch’s anemonefish (Amphiprion mccullochi) is endemic to a few oceanic reefs off Australia’s east coast a...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Springer
2012
|
| Online Access: | http://hdl.handle.net/20.500.11937/28041 |
| _version_ | 1848752429529563136 |
|---|---|
| author | van der Meer, M. Gardner, M. Hobbs, Jean-Paul Jones, G. van Herwerden, L. |
| author_facet | van der Meer, M. Gardner, M. Hobbs, Jean-Paul Jones, G. van Herwerden, L. |
| author_sort | van der Meer, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Endemic species at remote islands have a high risk of extinction because they often exhibit ecological, biological and genetic traits that make them particularly vulnerable to disturbances. McCulloch’s anemonefish (Amphiprion mccullochi) is endemic to a few oceanic reefs off Australia’s east coast and is an habitat specialist. Using 454 shotgun sequencing, we developed and evaluated primers for seventeen independent microsatellite loci to reveal gene flow, population genetic structure and genetic diversity across three isolated reefs. Observed and expected heterozygosities ranged from 0.556 to 1 and 0.543 to 0.907, respectively, in 30 Lord Howe Island individuals. When cross tested with the close relative, Amphiprion akindynos, amplification was successful with high levels of polymorphism. These loci will therefore be useful in studies of A. mccullochi, A. akindynos and possibly other closely related anemonefish. |
| first_indexed | 2025-11-14T08:08:29Z |
| format | Journal Article |
| id | curtin-20.500.11937-28041 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:08:29Z |
| publishDate | 2012 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-280412017-09-13T15:10:44Z Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi van der Meer, M. Gardner, M. Hobbs, Jean-Paul Jones, G. van Herwerden, L. Endemic species at remote islands have a high risk of extinction because they often exhibit ecological, biological and genetic traits that make them particularly vulnerable to disturbances. McCulloch’s anemonefish (Amphiprion mccullochi) is endemic to a few oceanic reefs off Australia’s east coast and is an habitat specialist. Using 454 shotgun sequencing, we developed and evaluated primers for seventeen independent microsatellite loci to reveal gene flow, population genetic structure and genetic diversity across three isolated reefs. Observed and expected heterozygosities ranged from 0.556 to 1 and 0.543 to 0.907, respectively, in 30 Lord Howe Island individuals. When cross tested with the close relative, Amphiprion akindynos, amplification was successful with high levels of polymorphism. These loci will therefore be useful in studies of A. mccullochi, A. akindynos and possibly other closely related anemonefish. 2012 Journal Article http://hdl.handle.net/20.500.11937/28041 10.1007/s12686-011-9517-1 Springer restricted |
| spellingShingle | van der Meer, M. Gardner, M. Hobbs, Jean-Paul Jones, G. van Herwerden, L. Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
| title | Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
| title_full | Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
| title_fullStr | Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
| title_full_unstemmed | Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
| title_short | Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
| title_sort | identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, amphiprion mccullochi |
| url | http://hdl.handle.net/20.500.11937/28041 |