Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen
Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
2001
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| Online Access: | http://hdl.handle.net/20.500.11937/27787 |
| _version_ | 1848752359803453440 |
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| author | Bindslev, L. Kershaw, M. Talbot, N. Oliver, Richard |
| author_facet | Bindslev, L. Kershaw, M. Talbot, N. Oliver, Richard |
| author_sort | Bindslev, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during infection-related development of the powdery mildew fungus Blumeria graminis (syn. Erysiphe graminis) f. sp. hordei, we described a gene similar to the catalytic subunit of cyclic AMP-dependent protein kinase A (here renamed Bka1). Functional characterization of this gene has been achieved by expression in a ΔcpkA mutant of the nonobligate pathogen Magnaporthe grisea. This nonpathogenic M. grisea ΔcpkA mutant displays delayed and incomplete appressorium development, suggesting a role for PKA-c in the signal transduction processes that control the maturation of infection cells. Transformation of the ΔcpkA mutant with the mildew Bka1 open reading frame, controlled by the M. grisea MPG1 promoter, restored pathogenicity and appressorium maturation kinetics. The results provide, to our knowledge, the first functional genetic analysis of pathogenicity in an obligate pathogen and highlight the remarkable conservation of signaling components regulating infection-related development in pathogenic fungi. |
| first_indexed | 2025-11-14T08:07:22Z |
| format | Journal Article |
| id | curtin-20.500.11937-27787 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:07:22Z |
| publishDate | 2001 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-277872017-09-13T15:55:52Z Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen Bindslev, L. Kershaw, M. Talbot, N. Oliver, Richard Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during infection-related development of the powdery mildew fungus Blumeria graminis (syn. Erysiphe graminis) f. sp. hordei, we described a gene similar to the catalytic subunit of cyclic AMP-dependent protein kinase A (here renamed Bka1). Functional characterization of this gene has been achieved by expression in a ΔcpkA mutant of the nonobligate pathogen Magnaporthe grisea. This nonpathogenic M. grisea ΔcpkA mutant displays delayed and incomplete appressorium development, suggesting a role for PKA-c in the signal transduction processes that control the maturation of infection cells. Transformation of the ΔcpkA mutant with the mildew Bka1 open reading frame, controlled by the M. grisea MPG1 promoter, restored pathogenicity and appressorium maturation kinetics. The results provide, to our knowledge, the first functional genetic analysis of pathogenicity in an obligate pathogen and highlight the remarkable conservation of signaling components regulating infection-related development in pathogenic fungi. 2001 Journal Article http://hdl.handle.net/20.500.11937/27787 10.1094/MPMI.2001.14.12.1368 restricted |
| spellingShingle | Bindslev, L. Kershaw, M. Talbot, N. Oliver, Richard Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen |
| title | Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen |
| title_full | Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen |
| title_fullStr | Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen |
| title_full_unstemmed | Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen |
| title_short | Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen |
| title_sort | complementation of the magnaporthe grisea cpka mutation by the blumeria graminis pka-c gene: functional genetic analysis of an obligate plant pathogen |
| url | http://hdl.handle.net/20.500.11937/27787 |