Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)

The majority of avian erythrocytes retain their nucleus throughout the circulatory lifespan of the cell; however, a small proportion of avian erythrocytes are enucleated to form erythroplastids. Previously, we have reported a Major Mitchell’s cockatoo that had a much greater proportion of erythropla...

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Main Authors: Clark, Phillip, Raidal, S.
Format: Journal Article
Published: Springer-Verlag London Ltd 2015
Online Access:http://hdl.handle.net/20.500.11937/27772
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author Clark, Phillip
Raidal, S.
author_facet Clark, Phillip
Raidal, S.
author_sort Clark, Phillip
building Curtin Institutional Repository
collection Online Access
description The majority of avian erythrocytes retain their nucleus throughout the circulatory lifespan of the cell; however, a small proportion of avian erythrocytes are enucleated to form erythroplastids. Previously, we have reported a Major Mitchell’s cockatoo that had a much greater proportion of erythroplastids than typically observed in avian blood. To further classify the erythrocytes of this bird, we employed fluorescent laser scanning confocal microscopy. Fluorescence of the cytoplasm of erythroid cells, including erythroplastids, facilitated assessment including z-stack analysis. Erythroplastids had a mean volume of 118 fL and were predominantly elliptical with minor depressions and infolding of cell surfaces.
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spelling curtin-20.500.11937-277722017-09-13T15:11:56Z Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri) Clark, Phillip Raidal, S. The majority of avian erythrocytes retain their nucleus throughout the circulatory lifespan of the cell; however, a small proportion of avian erythrocytes are enucleated to form erythroplastids. Previously, we have reported a Major Mitchell’s cockatoo that had a much greater proportion of erythroplastids than typically observed in avian blood. To further classify the erythrocytes of this bird, we employed fluorescent laser scanning confocal microscopy. Fluorescence of the cytoplasm of erythroid cells, including erythroplastids, facilitated assessment including z-stack analysis. Erythroplastids had a mean volume of 118 fL and were predominantly elliptical with minor depressions and infolding of cell surfaces. 2015 Journal Article http://hdl.handle.net/20.500.11937/27772 10.1007/s00580-015-2167-z Springer-Verlag London Ltd restricted
spellingShingle Clark, Phillip
Raidal, S.
Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)
title Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)
title_full Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)
title_fullStr Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)
title_full_unstemmed Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)
title_short Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos (Lophochroa leadbeateri)
title_sort confocal microscopic analysis of erythrocytes and erythroplastids from major mitchell’s cockatoos (lophochroa leadbeateri)
url http://hdl.handle.net/20.500.11937/27772