Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines
To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation prot...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Kluwer Academic Publishers
2014
|
| Online Access: | http://hdl.handle.net/20.500.11937/27481 |
| _version_ | 1848752275510525952 |
|---|---|
| author | Kanafi, M. Mamidi, M. Sureshbabu, S. Shahani, P. Bhawna, C. Warrier, Sudha Bhonde, R. |
| author_facet | Kanafi, M. Mamidi, M. Sureshbabu, S. Shahani, P. Bhawna, C. Warrier, Sudha Bhonde, R. |
| author_sort | Kanafi, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut-2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research. |
| first_indexed | 2025-11-14T08:06:02Z |
| format | Journal Article |
| id | curtin-20.500.11937-27481 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:06:02Z |
| publishDate | 2014 |
| publisher | Kluwer Academic Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-274812017-09-13T15:10:07Z Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines Kanafi, M. Mamidi, M. Sureshbabu, S. Shahani, P. Bhawna, C. Warrier, Sudha Bhonde, R. To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut-2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research. 2014 Journal Article http://hdl.handle.net/20.500.11937/27481 10.1007/s10529-014-1662-7 Kluwer Academic Publishers restricted |
| spellingShingle | Kanafi, M. Mamidi, M. Sureshbabu, S. Shahani, P. Bhawna, C. Warrier, Sudha Bhonde, R. Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| title | Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| title_full | Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| title_fullStr | Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| title_full_unstemmed | Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| title_short | Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| title_sort | generation of islet-like cell aggregates from human non-pancreatic cancer cell lines |
| url | http://hdl.handle.net/20.500.11937/27481 |