The immunological footprint of CMV in HIV-1 patients stable on long-term ART
BACKGROUND:Most HIV-infected persons are cytomegalovirus (CMV) seropositive and retain latent virus that can be reactivated by immune activation. Their T cell populations express markers reflecting a late stage of differentiation, but the contributions of HIV and CMV to this profile are unclear. We...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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2015
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| Online Access: | http://www.immunityageing.com/content/12/1/14 http://hdl.handle.net/20.500.11937/27354 |
| _version_ | 1848752240564633600 |
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| author | Affandi, J. Montgomery, J. Brunt, S. Nolan, D. Price, Patricia |
| author_facet | Affandi, J. Montgomery, J. Brunt, S. Nolan, D. Price, Patricia |
| author_sort | Affandi, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | BACKGROUND:Most HIV-infected persons are cytomegalovirus (CMV) seropositive and retain latent virus that can be reactivated by immune activation. Their T cell populations express markers reflecting a late stage of differentiation, but the contributions of HIV and CMV to this profile are unclear. We investigated the immunological "footprint" of CMV in HIV patients who had a history of extreme immunodeficiency but were now stable on antiretroviral therapy (ART).RESULTS:Twenty CMV seropositive HIV patients >50years old with nadir CD4 T-cell counts <200 cells/mul were studied after >12years on ART. 16 CMV seropositive and 9 CMV seronegative healthy controls were included. CMV antibody titres were higher in HIV patients than controls (P<0.001-0.003). Levels of soluble B-cell activating factor (sBAFF) were elevated in patients (P=0.002) and correlated with levels of CMV antibodies (P=0.03-0.002), with no clear relationship in controls. CD8 T-cell IFNgamma responses to the IE1 peptide (VLE) remained elevated in HIV patients (P=0.005). The CD57 + CD45RA + CD27 phenotype of CD8 T-cells correlated with age (r=0.60, P=0.006), antibodies against CMV IE1 protein (r=0.44, P=0.06) and CD4 T-cell IFNgamma response to CMV lysate (r=0.45, P=0.05).CONCLUSIONS:Humoral and T-cell responses to CMV remained elevated in HIV patients after >12years on ART. Age and presence of CMV disease influenced CD8 T-cell phenotypes. Elevated levels of sBAFF may be a consequence of HIV disease and contribute to high titres of CMV antibody. |
| first_indexed | 2025-11-14T08:05:29Z |
| format | Journal Article |
| id | curtin-20.500.11937-27354 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:05:29Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-273542017-09-13T15:32:24Z The immunological footprint of CMV in HIV-1 patients stable on long-term ART Affandi, J. Montgomery, J. Brunt, S. Nolan, D. Price, Patricia BACKGROUND:Most HIV-infected persons are cytomegalovirus (CMV) seropositive and retain latent virus that can be reactivated by immune activation. Their T cell populations express markers reflecting a late stage of differentiation, but the contributions of HIV and CMV to this profile are unclear. We investigated the immunological "footprint" of CMV in HIV patients who had a history of extreme immunodeficiency but were now stable on antiretroviral therapy (ART).RESULTS:Twenty CMV seropositive HIV patients >50years old with nadir CD4 T-cell counts <200 cells/mul were studied after >12years on ART. 16 CMV seropositive and 9 CMV seronegative healthy controls were included. CMV antibody titres were higher in HIV patients than controls (P<0.001-0.003). Levels of soluble B-cell activating factor (sBAFF) were elevated in patients (P=0.002) and correlated with levels of CMV antibodies (P=0.03-0.002), with no clear relationship in controls. CD8 T-cell IFNgamma responses to the IE1 peptide (VLE) remained elevated in HIV patients (P=0.005). The CD57 + CD45RA + CD27 phenotype of CD8 T-cells correlated with age (r=0.60, P=0.006), antibodies against CMV IE1 protein (r=0.44, P=0.06) and CD4 T-cell IFNgamma response to CMV lysate (r=0.45, P=0.05).CONCLUSIONS:Humoral and T-cell responses to CMV remained elevated in HIV patients after >12years on ART. Age and presence of CMV disease influenced CD8 T-cell phenotypes. Elevated levels of sBAFF may be a consequence of HIV disease and contribute to high titres of CMV antibody. 2015 Journal Article http://hdl.handle.net/20.500.11937/27354 10.1186/s12979-015-0041-0 http://www.immunityageing.com/content/12/1/14 unknown |
| spellingShingle | Affandi, J. Montgomery, J. Brunt, S. Nolan, D. Price, Patricia The immunological footprint of CMV in HIV-1 patients stable on long-term ART |
| title | The immunological footprint of CMV in HIV-1 patients stable on long-term ART |
| title_full | The immunological footprint of CMV in HIV-1 patients stable on long-term ART |
| title_fullStr | The immunological footprint of CMV in HIV-1 patients stable on long-term ART |
| title_full_unstemmed | The immunological footprint of CMV in HIV-1 patients stable on long-term ART |
| title_short | The immunological footprint of CMV in HIV-1 patients stable on long-term ART |
| title_sort | immunological footprint of cmv in hiv-1 patients stable on long-term art |
| url | http://www.immunityageing.com/content/12/1/14 http://hdl.handle.net/20.500.11937/27354 |