Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor

BACKGROUND The application of Chlorella vulgaris for simultaneous CO2 biofixation and nutrient removal has been optimised using response surface methodology (RSM) based on Box Behnken design (BBD). Experimental conditions employed comprised CO2 concentrations (Cc,g) of 0.03–22% CO2, irradiation inte...

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Main Authors: Al Ketife, Ahmed Majeed Daife, Judd, S., Znad, Hussein
Format: Journal Article
Published: Wiley 2016
Online Access:http://hdl.handle.net/20.500.11937/27109
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author Al Ketife, Ahmed Majeed Daife
Judd, S.
Znad, Hussein
author_facet Al Ketife, Ahmed Majeed Daife
Judd, S.
Znad, Hussein
author_sort Al Ketife, Ahmed Majeed Daife
building Curtin Institutional Repository
collection Online Access
description BACKGROUND The application of Chlorella vulgaris for simultaneous CO2 biofixation and nutrient removal has been optimised using response surface methodology (RSM) based on Box Behnken design (BBD). Experimental conditions employed comprised CO2 concentrations (Cc,g) of 0.03–22% CO2, irradiation intensities (I) of 100–400 μE, temperatures of 20–30 °C and nutrient concentrations of 0–56 and 0–19 mg L−1 nitrogen and phosphorus, respectively, the response parameters being specific growth rate μ, CO2 uptake rate Rc and %nutrient removal. RESULTS Over 10 days the biomass concentration reached almost 3 g L−1 for Cc,g of 5% CO2, with corresponding values of 0.74 g L−1 day−1 and 1.17 day−1 for Rc and μ, respectively, and 100% nutrient (N and P) removals. At 22% CO2 the Rc and μ decreased by around an order of magnitude, and nutrient removal also decreased to 79% and 50% for N and P, respectively. CONCLUSION Optimum values 5% CO2, 100 μE and 22 °C were identified for Cc,g, I and T, respectively, with μ and Rc reaching 1.53 day−1 and 1 g L−1 day−1, respectively, along with associated nutrient removal of 100%. Regression analysis indicated a good fit between experimental and model data. © 2016 Society of Chemical Industry
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spelling curtin-20.500.11937-271092017-09-13T15:30:51Z Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor Al Ketife, Ahmed Majeed Daife Judd, S. Znad, Hussein BACKGROUND The application of Chlorella vulgaris for simultaneous CO2 biofixation and nutrient removal has been optimised using response surface methodology (RSM) based on Box Behnken design (BBD). Experimental conditions employed comprised CO2 concentrations (Cc,g) of 0.03–22% CO2, irradiation intensities (I) of 100–400 μE, temperatures of 20–30 °C and nutrient concentrations of 0–56 and 0–19 mg L−1 nitrogen and phosphorus, respectively, the response parameters being specific growth rate μ, CO2 uptake rate Rc and %nutrient removal. RESULTS Over 10 days the biomass concentration reached almost 3 g L−1 for Cc,g of 5% CO2, with corresponding values of 0.74 g L−1 day−1 and 1.17 day−1 for Rc and μ, respectively, and 100% nutrient (N and P) removals. At 22% CO2 the Rc and μ decreased by around an order of magnitude, and nutrient removal also decreased to 79% and 50% for N and P, respectively. CONCLUSION Optimum values 5% CO2, 100 μE and 22 °C were identified for Cc,g, I and T, respectively, with μ and Rc reaching 1.53 day−1 and 1 g L−1 day−1, respectively, along with associated nutrient removal of 100%. Regression analysis indicated a good fit between experimental and model data. © 2016 Society of Chemical Industry 2016 Journal Article http://hdl.handle.net/20.500.11937/27109 10.1002/jctb.5084 Wiley restricted
spellingShingle Al Ketife, Ahmed Majeed Daife
Judd, S.
Znad, Hussein
Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor
title Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor
title_full Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor
title_fullStr Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor
title_full_unstemmed Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor
title_short Optimization of cultivation conditions for combined nutrient removal and CO2 fixation in a batch photobioreactor
title_sort optimization of cultivation conditions for combined nutrient removal and co2 fixation in a batch photobioreactor
url http://hdl.handle.net/20.500.11937/27109